Information on EC 1.2.2.3 - formate dehydrogenase (cytochrome-c-553)

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The expected taxonomic range for this enzyme is: Desulfovibrio

EC NUMBER
COMMENTARY hide
1.2.2.3
-
RECOMMENDED NAME
GeneOntology No.
formate dehydrogenase (cytochrome-c-553)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
formate + 2 ferricytochrome c-553 = CO2 + 2 ferrocytochrome c-553 + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
formate:ferricytochrome-c-553 oxidoreductase
Yeast cytochrome c, ferricyanide and phenazine methosulfate can act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
37251-01-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain G200, overproducing the enzyme
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Manually annotated by BRENDA team
strain Hildenborough
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formate + ferri-Ssc
CO2 + ferro-Ssc
show the reaction diagram
formate + ferri-TpIc3
CO2 + ferro-TpIc3
show the reaction diagram
formate + ferricytochrome c-553
CO2 + ferrocytochrome c-553
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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not: p-chloromercuribenzoate, idodoacetamide, sodium hypophosphite, EDTA or o-phenanthroline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
ferri-Ssc
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pH 7.6, 37°C
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0.0039
ferri-TpIc3
-
pH 7.6, 37°C
-
0.0027
Ferricytochrome c-553
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pH 7.6, 37°C
0.0074
formate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
27
ferri-Ssc
Desulfovibrio desulfuricans
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pH 7.6, 37°C
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17.1
ferri-TpIc3
Desulfovibrio desulfuricans
-
pH 7.6, 37°C
-
239
Ferricytochrome c-553
Desulfovibrio desulfuricans
-
pH 7.6, 37°C
1.13 - 1.92
formate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2100
ferri-Ssc
Desulfovibrio desulfuricans
-
pH 7.6, 37°C
42616
4400
ferri-TpIc3
Desulfovibrio desulfuricans
-
pH 7.6, 37°C
42615
88000
Ferricytochrome c-553
Desulfovibrio desulfuricans
-
pH 7.6, 37°C
9413
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.019
-
FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM lactate and 28 mM nitrate as electron acceptors, pH 7.6, 37°C
0.034
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FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM formate and 38 mM sulfate as electron acceptors, pH 7.6, 37°C
0.097
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FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM lactate and 38 mM sulfate as electron acceptors, pH 7.6, 37°C
0.14
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FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM formate and 28 mM nitrate as electron acceptors, pH 7.6, 37°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
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with K3Fe(CN)6 as acceptor
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10
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pH 4.5: about 40% of activity maximum, pH 10: about 10% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
one chromatography step
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
only the gamma subunit of the FDH2 complex (FDH2C) is cloned and expressed in Desulfovibrio desulfuricans
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
results indicate that FDH is more highly expressed in formate/nitrate-grown cells and that formate is a preferred electron donor for growth with nitrate, in contrast, the FDH activity of formate/sulfate-grown cells is low
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K62E
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mutant of cytochrome c553 per site-directed mutagenesis with 3fold increased initial reaction velocity
K62E/K63E
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mutant of cytochrome c553 per site-directed mutagenesis with decreased turnover and recognition of the enzyme, but 5fold increased initial reaction velocity
Y64A
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detailed kinetics, reduced electron exchange with enzyme
Y64F
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detailed kinetics, reduced electron exchange with enzyme
Y64L
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detailed kinetics, reduced electron exchange with enzyme
Y64V
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detailed kinetics, reduced electron exchange with enzyme
Y64A
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detailed kinetics, reduced electron exchange with enzyme
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Y64F
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detailed kinetics, reduced electron exchange with enzyme
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Y64L
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detailed kinetics, reduced electron exchange with enzyme
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Y64V
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detailed kinetics, reduced electron exchange with enzyme
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