Information on EC 1.2.1.82 - beta-apo-4'-carotenal oxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.82
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RECOMMENDED NAME
GeneOntology No.
beta-apo-4'-carotenal oxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4'-apo-beta,psi-caroten-4'-al + NAD+ + H2O = neurosporaxanthin + NADH + 2 H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
neurosporaxanthin biosynthesis
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Carotenoid biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
4'-apo-beta,psi-carotenal:NAD+ oxidoreductase
Neurosporaxanthin is responsible for the orange color of of Neurospora.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
apo-4'-lycopenal + NAD+ + H2O
apo-4'-lycopenoic acid + NADH + 2 H+
show the reaction diagram
the in vivo substrate of Ylo-1 is apo-4'-lycopenal rather than its cyclic isomer beta-apo-4'-carotenal
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?
apo-8'-lycopenal + NAD+ + H2O
apo-8'-lycopenoic acid + NADH + 2 H+
show the reaction diagram
apo-8'-lycopenal + NAD+ + H2O
apo-8'-lycopenoic acid + NADH + H+
show the reaction diagram
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?
beta-apo-10'-carotenal + NAD+ + H2O
beta-apo-10'-carotenoic acid + NADH + 2 H+
show the reaction diagram
beta-apo-10'-carotenal + NAD+ + H2O
beta-apo-10'-carotenoic acid + NADH + H+
show the reaction diagram
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?
beta-apo-4'-carotenal + NAD+ + H2O
beta-apo-4'-carotenoic acid + NADH + H+
show the reaction diagram
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
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?
beta-apo-4'-carotenal + NAD+ + H2O
neurosporaxanthin + NADH + 2 H+
show the reaction diagram
beta-apo-8'-carotenal + NAD+ + H2O
apo-8'-carotenoic acid + NADH + 2 H+
show the reaction diagram
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?
beta-apo-8'-carotenal + NAD+ + H2O
beta-apo-8'-carotenoic acid + NADH + 2 H+
show the reaction diagram
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?
beta-apo-8'-carotenal + NAD+ + H2O
beta-apo-8'-carotenoic acid + NADH + H+
show the reaction diagram
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-apo-4'-carotenal + NAD+ + H2O
beta-apo-4'-carotenoic acid + NADH + H+
show the reaction diagram
F6IBC7
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
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?
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification by generating of a crude lysate for in vitro assays
recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
carD expressed in Escherichia coli BL21 strain; generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants; carD mRNA levels increase about 3fold after 30 min of illumination
Ylo-1 mRNA is not induced by light
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