Information on EC 1.2.1.78 - 2-formylbenzoate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.78
-
RECOMMENDED NAME
GeneOntology No.
2-formylbenzoate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-formylbenzoate + NAD+ + H2O = o-phthalic acid + NADH + H+
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Polycyclic aromatic hydrocarbon degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
2-formylbenzoate:NAD+ oxidoreductase
The enzyme is involved in phenanthrene degradation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain AFK2
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-
Manually annotated by BRENDA team
strain AFK2
-
-
Manually annotated by BRENDA team
strain KP7
SwissProt
Manually annotated by BRENDA team
strain KP7
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-carboxybenzaldehyde + NAD+ + H2O
o-phthalic acid + NADH + H+
show the reaction diagram
2-nitrobenzaldehyde + NAD+ + H2O
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-carboxybenzaldehyde + NAD+ + H2O
o-phthalic acid + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-naphthoaldehyde
-
0.2 mM, 34.6% inhibition
4-carboxybenzaldehyde
-
0.2 mM, 38.5% inhibition
acetaldehyde
-
0.2 mM, 34.6% inhibition
Ag+
-
1 mM, complete inhibition
Ca2+
10 mM, about 30% inhibition
Co2+
-
1 mM, 40.2% inhibition
Cu2+
-
1 mM, complete inhibition
EDTA
10 mM, 28% inhibition
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid
10 mM, 14% inhibition
Hg2+
-
1 mM, 17.5% inhibition
iso-phthalaldehyde
-
0.2 mM, 23.8% inhibition
Mg2+
10 mM, about 30% inhibition
Mn2+
10 mM, about 30% inhibition
o-nitrobenzaldehyde
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0.2 mM, 58.1% inhibition
o-phthalaldehyde
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0.2 mM, 91.4% inhibition
p-chloromercuribenzoate
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1 mM, complete inhibition
p-dimethylamino benzaldehyde
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0.2 mM, 32.7% inhibition
propionaldehyde
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0.2 mM, 31.7% inhibition
Protocatechualdehyde
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0.2 mM, 23.1% inhibition
Salicylaldehyde
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0.2 mM, 23.1% inhibition
SDS
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1 mM, 44.3% inhibition
vertralaldehyde
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0.2 mM, 13.5% inhibition
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Zn2+
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1 mM, 71.7% inhibition
additional information
less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054 - 2.8
2-carboxybenzaldehyde
0.083 - 0.14
NAD+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39
2-carboxybenzaldehyde
Nocardioides sp.
Q79EM7
pH 9.4, 25C
48
2-nitrobenzaldehyde
Nocardioides sp.
Q79EM7
pH 9.4, 25C
32
NAD+
Nocardioides sp.
Q79EM7
pH 9.4, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.6
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pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
158000
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gel filtration
205000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
10 min, no loss of activity below 30C
40
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10 min, 25% loss of activity
50
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10 min, 94% loss of activity
60
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10 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, activity in phosphate buffer is labile, 1 mM DTT stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli