Information on EC 1.2.1.7 - benzaldehyde dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.2.1.7
-
RECOMMENDED NAME
GeneOntology No.
benzaldehyde dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
benzaldehyde + NADP+ + H2O = benzoate + NADPH + 2 H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,5-xylenol and 3,5-xylenol degradation
-
-
Aminobenzoate degradation
-
-
m-cresol degradation
-
-
mandelate degradation I
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
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-
patulin biosynthesis
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Toluene degradation
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-
Xylene degradation
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4-hydroxymandelate degradation
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-
SYSTEMATIC NAME
IUBMB Comments
benzaldehyde:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-89-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
flounder
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strain K172, formerly Pseudomonas strain K172
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dihydroxybenzaldehyde + NAD+ + H2O
2,4-dihydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
3,4-dihydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O
3-hydroxy-4-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
isovanillin + NADP+ + H2O
3-hydroxy-4-methoxybenzoate + NADPH
show the reaction diagram
-
-
-
-
?
salicylaldehyde + NADP+ + H2O
salicylic acid + NADPH
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Disulfiram
N-ethylmaleimide
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-
p-hydroxymercuribenzoate
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
K+
-
125 mM KCl, 1.5 fold increase in activity
Na+
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125 mM NaCl, 1.5 fold increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037
3,4-Dihydroxybenzaldehyde
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apparent KM
0.044
4-hydroxy-3-methoxybenzaldehyde
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apparent KM
0.006
4-hydroxybenzaldehyde
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apparent KM
0.0002 - 0.1
benzaldehyde
0.6
NAD+
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apparent KM
0.037 - 0.13
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25
3,4-Dihydroxybenzaldehyde
Acinetobacter baylyi
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apparent Kcat
18
4-hydroxy-3-methoxybenzaldehyde
Acinetobacter baylyi
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apparent Kcat
4
4-hydroxybenzaldehyde
Acinetobacter baylyi
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apparent Kcat
4.6
benzaldehyde
Acinetobacter baylyi
-
apparent Kcat
14
NAD+
Acinetobacter baylyi
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apparent Kcat
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0086
-
4-hydroxybenzaldehyde and also benzaldehyde
0.034
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cells grown on benzoate
0.065
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cells grown on benzyl alcohol
0.091
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cells grown on toluene
0.132
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cells grown on benzaldehyde
0.28
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cells grown on D-phenylglycine
0.3
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cells grown on L-phenylglycine
3.2
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clarified lysate, total activity 37 U, protein concentration 5.77 mg/ml
22
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IMAC eluted 4-hydroxy benzaldehyde dehydrogenase, total activity 35 Units, protein concentration 0.27 mg/ml
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9 - 10.7
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the activities measured at pH 6.85 and 10.7 are approximately 30% of the activity measured at pH 9.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21
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activity assay
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21 - 45
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21C 100%, 37C 90%, 45C 75% activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
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determined by isoelectric focusing electrophoresis
5.8
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predicted from the amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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determined by SDS-PAGE
56300
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4 * 56300, SDS-PAGE
116000
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determined by gel filtration
122000
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gel filtration
200000
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sucrose density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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? x 57000, sequence determination
homodimer
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2 * 55000
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
inactivation after 5 min
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-17C, pH 7.2, 10 mM dithiothreitol, several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
on a His-Trap FF IMAC column
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli K12 derivatives
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into the vector pET-Duet1 for expression in Escherichia coli BL21DE3 cells
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restriction mapping of native plasmids, subcloning of the operon in Escherichia coli and Pseudomonas sp.
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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random mutagenesis of plasmid encoded benzaldehyde dehydrogenase gene xylC, expression in Escherichia coli