Information on EC 1.2.1.69 - fluoroacetaldehyde dehydrogenase

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The expected taxonomic range for this enzyme is: Streptomyces cattleya

EC NUMBER
COMMENTARY hide
1.2.1.69
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RECOMMENDED NAME
GeneOntology No.
fluoroacetaldehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
fluoroacetaldehyde + NAD+ + H2O = fluoroacetate + NADH + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
fluoroacetate and fluorothreonine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
fluoroacetaldehyde:NAD+ oxidoreductase
The enzyme from Streptomyces cattleya has a high affinity for fluoroacetate and glycolaldehyde but not for acetaldehyde.
CAS REGISTRY NUMBER
COMMENTARY hide
387336-50-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
DL-glyceraldehyde + NAD+ + H2O
DL-glycerate + NADH + H+
show the reaction diagram
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10% of activity with fluoroacetaldehyde
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?
fluroracetaldehyde + NAD+ + H2O
fluoroacetate + NADH
show the reaction diagram
fluroracetaldehyde + NAD+ + H2O
fluoroacetate + NADH + H+
show the reaction diagram
glycoaldehyde + NAD+ + H2O
glycolate + NADH + H+
show the reaction diagram
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150% of activity with fluoroacetaldehyde
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?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
fluroracetaldehyde + NAD+ + H2O
fluoroacetate + NADH
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chloroacetaldehyde
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0.25 mM, time-dependent inactivation
Cu2+
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1 mM, strong inhibition
iodoacetamide
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0.05 mM, strong inhibition
Mg2+
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1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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1 mM, increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.81
acetaldehyde
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0.32
benzaldehyde
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0.08
fluoroacetaldehyde
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0.15
glycoaldehyde
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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4 * 55000, SDS-PAGE
200000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, anion-exchange, 5'-AMP-agarose
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