Information on EC 1.2.1.65 - salicylaldehyde dehydrogenase

Word Map on EC 1.2.1.65
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.65
-
RECOMMENDED NAME
GeneOntology No.
salicylaldehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
salicylaldehyde + NAD+ + H2O = salicylate + NADH + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
Naphthalene degradation
-
-
naphthalene degradation (aerobic)
-
-
SYSTEMATIC NAME
IUBMB Comments
salicylaldehyde:NAD+ oxidoreductase
Involved in the naphthalene degradation pathway in some bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
55354-34-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PpG1064
-
-
Manually annotated by BRENDA team
strain NGK1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-chlorosalicylaldehyde + NAD+ + H2O
5-chlorosalicylate + NADH + H+
show the reaction diagram
caprylic aldehyde + NAD+ + H2O
caprylic acid + NADH + H+
show the reaction diagram
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
glutaraldehyde + NAD+ + H2O
glutarate + NADH + H+
show the reaction diagram
glyoxal + NAD+ + H2O
glyoxylate + NADH + H+
show the reaction diagram
m-nitrobenzaldehyde + NAD+ + H2O
m-nitrobenzoate + NADH + H+
show the reaction diagram
-
-
-
?
o-methoxybenxaldehyde + NAD+ + H2O
o-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
o-nitrobenzoate + NADH + H+
show the reaction diagram
-
-
-
?
salicylaldehyde + NAD+ + H2O
salicylate + NADH
show the reaction diagram
salicylaldehyde + NAD+ + H2O
salicylate + NADH + 2 H+
show the reaction diagram
salicylaldehyde + NAD+ + H2O
salicylate + NADH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
salicylaldehyde + NAD+ + H2O
salicylate + NADH
show the reaction diagram
additional information
?
-
-
involved in the degradation of acenaphthylene
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
enhances NahV activity by 63% activity
Fe2+
enhances NahF activity by 40% activity; enhances NahV activity by 152%
Zn2+
enhances NahV activity by 14% activity
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
inhibits NahF activity by 42%; inhibits NahV activity by 15%
Ba2+
inhibits NahF activity by 62%; inhibits NahV activity by 52%
Ca2+
inhibits NahF activity by 63%; inhibits NahV activity by 35%
Cs+
inhibits NahF activity by 44%; inhibits NahV activity by 69%
Cu2+
inhibits NahF activity by 70%
Li+
inhibits NahF activity by 50%; inhibits NahV activity by 57%
Mg2+
inhibits NahF activity by 34%; inhibits NahV activity by 36%
NADP+
-
7% inhibition at 5 mM with 5 mM NAD+
Ni2+
inhibits NahF activity by 60%; inhibits NahV activity by 70%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00016 - 0.15
NAD+
0.034 - 0.146
Salicylaldehyde
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
-
in cell free extract of Stenotrophomonas sp. RMSK grown on 1-naphthoic acid
0.23
-
in cell free extract of Stenotrophomonas sp. RMSK grown on acenaphthylene
0.89
-
wild type naphthalene-succinate grown cells
1.33
-
salicylate-induced enzyme synthesis
1.36
-
salicylate-induced enzyme synthesis
1.8
-
strain PpG7, salicylate-induced enzyme synthesis
15
-
nahF mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
67.81
-
nahV mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
85.4
-
wild-type ND6 with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C
118
-
mutant strain MC4, 2-aminobenzoate induced cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
1 * 50000, SDS-PAGE; 1 * 50000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals of recombinant 6xHis-NahF obtained at 291 K and diffracted to 2.42 A resolution
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
72.8% activity is retained for NahF after a 30 min exposure; NahV looses 88.8% activity after a 30 min exposure
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by Ni2+ affinity chromatography followed by gel filtration
-
cell free extracts are prepared
-
His-tagged NahF purified by Ni-NTA chromatography; His-tagged NahV purified by Ni-NTA chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli and Pseudomonas aeruginosa
-
mutant strains, lacking induction of enzyme synthesis by naphthalene growth media
-
nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells
-
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3); nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a 10fold increase in nahV expression observed in the ND6-NahF mutant when cultured in the presence of naphthalene and a 30fold increase when cultured in the presence of salicylate; nahF expression levels in the ND6-NahV mutant elevated by 20fold compared to the levels observed in the wild-type strain cultured in the presence of naphthalene and a 5fold increase when cultured in the presence of salicylate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
environmental protection
-
the ability to degrade acenaphthylene and other aromatic compounds makes this strain ideal candidate for application in remediation at the contaminated sites
additional information