Information on EC 1.2.1.5 - aldehyde dehydrogenase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.2.1.5
-
RECOMMENDED NAME
GeneOntology No.
aldehyde dehydrogenase [NAD(P)+]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-Alanine metabolism
-
-
Biosynthesis of secondary metabolites
-
-
Drug metabolism - cytochrome P450
-
-
Glycolysis / Gluconeogenesis
-
-
Histidine metabolism
-
-
L-tryptophan degradation V (side chain pathway)
-
-
Metabolic pathways
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-
Metabolism of xenobiotics by cytochrome P450
-
-
Microbial metabolism in diverse environments
-
-
Phenylalanine metabolism
-
-
Tyrosine metabolism
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
aldehyde:NAD(P)+ oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-88-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Bo
-
-
Manually annotated by BRENDA team
strain Bo
-
-
Manually annotated by BRENDA team
strain LB400
Uniprot
Manually annotated by BRENDA team
; strain 103
-
-
Manually annotated by BRENDA team
strain 103
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
subspecies Zea mays mays
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-dihydroxyacetone + NAD+ + H2O
? + NADH + H+
show the reaction diagram
53.93% of the activity compared to acetaldehyde
-
-
?
1-formyl-6-methylpyrene + NADP+ + H2O
6-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formyl-8-methylpyrene + NADP+ + H2O
8-methylpyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
1-formylpyrene + NADP+ + H2O
pyrene-1-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2,5-diyhydroxybenzaldehyde + NADP+ + H2O
2,5-dihydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
2-bromobenzaldehyde + NAD+ + H2O
2-bromobenzoate + NADH + H+
show the reaction diagram
2-chlorobenzaldehyde + NAD+ + H2O
2-chlorobenzoate + NADH + H+
show the reaction diagram
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
show the reaction diagram
2-formylpyrene + NADP+ + H2O
pyrene-2-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
2-methylbutyraldehyde + NAD+ + H2O
2-methylbutyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NADH + H+
2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxymandelaldehyde + NAD+ + H2O
3,4-dihydroxyphenylmandelate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+
3,4-dihydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
?
3,4-dihydroxyphenylglycolaldehyde + NAD+ + H2O
3,4-dihydroxyphenylglycolate + NADH + H+
show the reaction diagram
-
-
-
?
3-hydroxybenzaldehyde + NADP+ + H2O
3-hydroxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropanoate + NADH + 2 H+
show the reaction diagram
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
show the reaction diagram
32.98% of the activity compared to acetaldehyde
-
-
?
4-bromobenzaldehyde + NAD+ + H2O
4-bromobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-carboxybenzaldehyde + NADP+ + H2O
4-carboxybenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADP+ + H2O
4-chlorobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NADP+ + H2O
pyrene-4-carboxylic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
4-nitrobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-pyridinecarboxaldehyde + NAD+ + H2O
4-pyridinecarboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
5-hydroxyindole-3-acetyldehyde + NAD+ + H2O
5-hydroxyindole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NADH + H+
6-methoxy-2-naphthyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
acetaldehyde + 3-pyridine aldehyde adenine dinucleotide phosphate
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + acetylpyridine adenine dinucleotide
acetate + ?
show the reaction diagram
-
-
-
-
?
acetaldehyde + deamino-NAD+ + H2O
acetate + deamino-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
acetaldehyde + NAD+
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
acetaldehyde + NADP+
acetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH
show the reaction diagram
-
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH + H+
show the reaction diagram
acetaldehyde + thio-NAD+ + H2O
acetate + thio-NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + thio-NADP+
acetate + ?
show the reaction diagram
-
-
-
-
?
aldophosphamide + NAD+
N,N-bis-(2-chloroethyl)-diaminophosphoric-(2-carboxyethylester) + ?
show the reaction diagram
-
-
-
-
?
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NADP+
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH + H+
show the reaction diagram
butyraldehyde + NAD(P)+ + H2O
butanoate + NAD(P)H + H+
show the reaction diagram
butyraldehyde + NAD+
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
butyraldehyde + NADP+
butyrate + NADPH + H+
show the reaction diagram
-
-
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + H+
show the reaction diagram
-
-
-
-
?
cinnamaldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
-
-
-
-
?
D-glyceraldehyde + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
50.25% of the activity compared to acetaldehyde. kcat/KM for NAD+ with D-glyceraldehyde as cosubstrate is 3.8fold higher compared to NADP+
-
-
?
D-glyceraldehyde + NADP+ + H2O
D-glycerate + NADPH
show the reaction diagram
50.25% of the activity compared to acetaldehyde
-
-
?
D-glyceraldehyde 3-phosphate + NAD+ + H2O
D-glycerate 3-phosphate + NADH + H+
show the reaction diagram
52.7% of the activity compared to acetaldehyde
-
-
?
DL-glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
-
14% of activity with benzaldehyde
-
-
ir
dodecanal + NADP+ + H2O
dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD(P)+ + H2O
formate + NAD(P)H + H+
show the reaction diagram
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
formaldehyde + NADP+ + H2O
formate + NADPH + H+
show the reaction diagram
furfuryl aldehyde + NAD+ + H2O
2-furancarboxylate + NADH + H+
show the reaction diagram
glutaraldehyde + NAD+
glutarate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD(P)+ + H2O
glycerate + NAD(P)H + H+
show the reaction diagram
-
-
-
ir
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+
glycolate + NADH
show the reaction diagram
glycolaldehyde + NAD+ + H2O
glycolate + NADH + H+
show the reaction diagram
75.43% of the activity compared to acetaldehyde
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
show the reaction diagram
heptanal + NADP+ + H2O
heptanoate + NADPH + H+
show the reaction diagram
-
-
-
?
heptylaldehyde + NAD(P)+ + H2O
heptanoate + NAD(P)H + H+
show the reaction diagram
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
hexylaldehyde + NAD(P)+ + H2O
hexanoate + NAD(P)H + H+
show the reaction diagram
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
?
isobutanal + NAD+ + H2O
isobutyrate + NADH + H+
show the reaction diagram
isobutyraldehyde + NAD(P)+ + H2O
isobutanoate + NAD(P)H + H+
show the reaction diagram
isopentanal + NAD+ + H2O
isopentanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NAD(P)+ + H2O
isovalerate + NAD(P)H
show the reaction diagram
isovaleraldehyde + NAD+
isovalerate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NADP+
isovalerate + NADPH + H+
show the reaction diagram
isovaleraldehyde + NADP+ + H2O
isovalerate + NADPH + H+
show the reaction diagram
-
-
-
-
m-bromobenzaldehyde + NAD(P)+ + H2O
m-bromobenzoate + NAD(P)H + H+
show the reaction diagram
m-chlorobenzaldehyde + NAD(P)+ + H2O
m-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
m-fluorobenzaldehyde + NAD(P)+ + H2O
m-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
mafosfamide + NADP+ + H2O
?
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+
? + NADH
show the reaction diagram
malonic semialdehyde + NAD+
?
show the reaction diagram
-
-
-
-
?
n-butanal + NAD+ + H2O
butanoate + NADH + H+
show the reaction diagram
n-butanal + NADP+ + H2O
n-butanoate + NADPH + H+
show the reaction diagram
n-decanal + NADP+ + H2O
n-decanoate + NADPH + H+
show the reaction diagram
n-dodecanal + NADP+ + H2O
n-dodecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-hexanal + NADP+ + H2O
n-hexanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-nonanal + NADP+ + H2O
n-nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-octanal + NADP+ + H2O
n-octanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-pentanal + NAD+ + H2O
n-pentanoate + NADH + H+
show the reaction diagram
n-pentanal + NADP+ + H2O
n-pentanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+
propionate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-propanal + NADP+ + H2O
n-propanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tetradecanal + NADP+ + H2O
n-tetradecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-tridecanal + NADP+ + H2O
n-tridecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
n-undecanal + NADP+ + H2O
n-undecanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
nonanal + NADP+ + H2O
nonanoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
o-fluorobenzaldehyde + NAD(P)+ + H2O
o-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
o-methoxybenzaldehyde + NAD(P)+ + H2O
o-methoxybenzoate + NAD(P)H + H+
show the reaction diagram
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
octylaldehyde + NAD(P)+ + H2O
octanoate + NAD(P)H + H+
show the reaction diagram
p-bromobenzaldehyde + NAD(P)+ + H2O
p-bromobenzoate + NAD(P)H + H+
show the reaction diagram
p-chlorobenzaldehyde + NAD(P)+ + H2O
p-chlorobenzoate + NAD(P)H + H+
show the reaction diagram
p-fluorobenzaldehyde + NAD(P)+ + H2O
p-fluorobenzoate + NAD(P)H + H+
show the reaction diagram
p-hydroxybenzaldehyde + NADP+ + H2O
p-hydroxybenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
-
?
p-isopropylbenzaldehyde + NAD(P)+ + H2O
p-isopropylbenzoate + NAD(P)H + H+
show the reaction diagram
p-nitrobenzaldehyde + NADP+ + H2O
p-nitrobenzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NADP+ + H2O
phenylacetate + NADPH + H+
show the reaction diagram
-
-
-
-
?
polyethylene glycol 1000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 200-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 400-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
polyethylene glycol 4000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
highest activtiy compared to other polyethylene glycolaldehydes
-
-
?
polyethylene glycol 6000-aldehyde + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
propanal + 1,N6-ethenoadenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-acetylpyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-pyridine adenine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + 3-thionicotinamide adenine dinucleotide
propionate + ?
show the reaction diagram
propanal + beta-NADP+ + H2O
propionate + beta-NADPH + H+
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-guanine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + N-hypoxanthine dinucleotide
propionate + ?
show the reaction diagram
-
isoenzyme E1 and E2
-
-
?
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NAD(P)+ + H2O
propionate + NAD(P)H
show the reaction diagram
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
propionaldehyde + NADP+ + H2O
propionate + NADPH + H+
show the reaction diagram
retinal + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
preferred substrate of isozyme ALDH1A3
-
-
?
succinic semialdehyde + NAD+
? + NADH
show the reaction diagram
-
-
-
-
?
trans-2-hexenal + NAD+
trans-2-hexenoate + NADH
show the reaction diagram
valeraldehyde + NAD(P)+ + H2O
valerate + NAD(P)H + H+
show the reaction diagram
valeraldehyde + NAD+
valerate + NADH + H+
show the reaction diagram
-
-
-
-
?
valeraldehyde + NADP+
valerate + NADPH + H+
show the reaction diagram
valeraldehyde + NADP+ + H2O
valerate + NADPH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxy-2-nonenal + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
ir
an aldehyde + NAD(P)+ + H2O
an acid + NAD(P)H + H+
show the reaction diagram
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
; stimulating
CaCl2
-
30 mM, 3.8fold stimulation of activity of ALDH1
KCl
-
120 mM: 40fold increase of activity of ALDH5 with NAD+. 16 mM: 20fold increase of activity of enzyme ALDH5 with NADP+. No stimulation of the activity of enzyme form ALDH1
Li+
500 mM, 107% of the activity compared to activity without metal ions
MnCl2
-
2 mM, 2.1fold stimulation of activity of ALDH1
Na+
500 mM, 1.2fold activation
additional information
-
the enzyme does not require metal ions for its enzymatic reaction
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Cyclohexanedione
-
33% residual activity at 2.5 mM
2,4-Dinitro-1-fluorobenzene
-
11% residual activity at 2.5 mM
2-mercaptoethanol
10% (v/v), 55% inhibition
3-thionicotinamide adenine dinucleotide
-
strong inhibition versus beta-NAD+, isoenzyme E2; substrate inhibition, isoenzyme E1
acetaldehyde
-
strong substrate inhibition
acetone
10% (v/v), 21% inhibition
benomyl
potent irreversible inhibitor of ALDH. Benomyl increases superoxide production as much as nitroglycerin
Benzoate
-
non-competitive with respect to either NAD+ or benzaldehyde
beta-NADP+
-
substrate inhibition, isoenzyme E1
Cd2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
Chloral hydrate
Co2+
-
89% residual activity at 1 mM
diethyl dicarbonate
-
2% residual activity at 2.5 mM
dimethyl ampal thiolester
-
complete inhibition of isozyme ALDH3 at 0.0075 mM
Disulfiram
DMSO
10% (v/v), 32% inhibition
EDTA
50 mM, 22% inhibition
ethanediol
10% (v/v), 60% inhibition
ethyl acetimidate
-
71% residual activity at 250 mM
Fe2+
-
88% residual activity at 1 mM
guanidine hydrochloride
10% (v/v), 42% inhibition
H2O2
the loss of activity induced by H2O2 can be restored by reducing agents, although a decrease of the restored activity is observed upon addition of 10 mM dithiothreitol
methyl ampal thiolester
-
78% inhibition of isozyme ALDH3 at 0.0075 mM
Mg2+
-
89% residual activity at 1 mM
Mn2+
-
89% residual activity at 1 mM
N-Acetylimidazole
-
75% residual activity at 2.5 mM
N-bromosuccinimide
-
66% residual activity at 0.5 mM
nitroglycerin
p-chloromercuribenzoate
-
50 microM, 80% inhibition
p-hydroxymercuribenzoate
p-mercuribenzoate
-
80% inhibition at 0.05 mM
Pb2+
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
PEG-400
10% (v/v), 16% inhibition
pentanal
-
extremely strong substrate inhibition
phenylmethylsulfonyl fluoride
-
73% residual activity at 2.5 mM
PMSF
50 mM, no loss of activity
pyrazole
-
100% inhibition at 1 mM; 1 mM, 100% inhibition
trans-4-(N,N-dimethylamino)-cinnamaldehyde
-
1 mM, 58% inhibition; 58% inhibition at 1 mM
Urea
500 mM, 44% inhibition
Woodward's reagent K
-
71% residual activity at 50 mM
ZnCl2
-
50% inhibition at 0.035 mM, enzyme form ALDH1
additional information
-
not inhibited by EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
246% activity at 2 mM
dithiothreitol
-
276% activity at 2 mM
ethanol
-
0.05 mM, 1.8fold stimulation
H2O2
-
0.015 mM, 1.2fold stimulation
NH4+
500 mM, 1.31fold activation
Sodium dodecyl sulfate
-
0.01 mM, 1.1fold stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
1,N6-ethenoadenine dinucleotide
-
isoenzyme E2
0.0016
1-formyl-6-methylpyrene
-
ALDH3A1, at pH 7.5 and 37C
0.004
1-formyl-8-methylpyrene
-
ALDH3A1,at pH 7.5 and 37C
0.004
1-formylpyrene
-
ALDH3A1, at pH 7.5 and 37C
0.012 - 0.379
2-Bromobenzaldehyde
0.006 - 0.063
2-Chlorobenzaldehyde
0.008 - 0.822
2-Fluorobenzaldehyde
0.011
2-formylpyrene
-
ALDH3A1, at pH 7.5 and 37C
0.0007
2-naphthaldehyde
-
pH 7.5, 25C
0.39
3,4-dihydroxyphenyl acetaldehyde
-
reaction with NAD+
0.61
3,4-dihydroxyphenyl benzaldehyde
-
reaction with NAD+
0.0004 - 0.015
3,4-dihydroxyphenylacetaldehyde
0.0132 - 0.62
3-acetylpyridine adenine dinucleotide
0.0338 - 0.504
3-pyridinealdehyde adenine dinucleotide
0.0151
3-thionicotinamide adenine dinucleotide
-
isoenzyme E1
0.039 - 0.617
4-bromobenzaldehyde
0.031 - 1.159
4-Chlorobenzaldehyde
0.012 - 2.057
4-Fluorobenzaldehyde
0.00078
4-formylpyrene
-
ALDH3A1, at pH 7.5 and 37C
0.03
4-hydroxynonenal
-
-
0.081 - 1.103
4-iodobenzaldehyde
0.091 - 0.19
4-Pyridinecarboxaldehyde
0.15
5-hydroxyindole-3-acetaldehyde
-
reaction with NAD+
0.0033
6-methoxy-2-naphthaldehyde
-
pH 7.5, 25C
0.0002 - 393.4
acetaldehyde
0.64
aldophosphamide
-
reaction with NAD+
0.013 - 4.15
benzaldehyde
0.0047
beta-NAD+
-
-
7.68
beta-NADP+
-
isoenzyme E1
0.094
betaNAD+
-
isoenzyme E2
2.95 - 4.93
Butyraldehyde
0.01 - 0.46
Chloroacetaldehyde
0.0053
cinnamaldehyde
-
pH 7.5, 25C
8.51
D-glyceraldehyde
pH 8, 80C
6.12
D-glyceraldehyde 3-phosphate
pH 8, 80C
0.27 - 1.9
formaldehyde
0.05 - 0.13
glycolaldehyde
0.06 - 0.08
Hexanal
0.5
Indole-3-acetaldehyde
-
reaction with NAD+
0.016
Isobutyraldehyde
30C, pH 10.0
0.0005
isopentanal
-
isoenzyme F1
1.66 - 10.78
Isovaleraldehyde
0.0113
N-1,N6-ethenoadenine dinucleotide
-
isoenzyme E1
0.137
n-dodecanal
-
50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36C
1.33
N-guanine dinucleotide
-
isoenzyme E1
0.121 - 1.36
N-hypoxanthine dinucleotide
0.003 - 35
NAD+
0.01 - 260
NADP+
0.067 - 0.113
octanal
0.0001
pentanal
-
isoenzyme F1
0.0003 - 0.004
phenylacetaldehyde
0.00039 - 19.06
propanal
0.46 - 12
propionaldehyde
0.1 - 0.12
trans-2-hexenal
0.12 - 0.34
Valeraldehyde
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.1
4-hydroxynonenal
Mus musculus
-
-
1.11 - 35.9
acetaldehyde
20.9 - 51
benzaldehyde
11.5 - 32.8
Butyraldehyde
0.47 - 7.7
Chloroacetaldehyde
0.92
D-glyceraldehyde
Sulfolobus tokodaii
Q976X5
pH 8, 80C
0.83
D-glyceraldehyde 3-phosphate
Sulfolobus tokodaii
Q976X5
pH 8, 80C
34
Hexanal
0.91 - 3.58
Isovaleraldehyde
0.47 - 45.3
NAD+
3.71 - 38.8
NADP+
13.36 - 49.38
propionaldehyde
35.1 - 53
trans-2-hexenal
19.41 - 30.99
Valeraldehyde
additional information
additional information
Rattus norvegicus
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.24
acetaldehyde
90
2.39 - 11.89
Butyraldehyde
499
5.5 - 200
Chloroacetaldehyde
2475
0.11
D-glyceraldehyde
Sulfolobus tokodaii
Q976X5
pH 8, 80C
639
0.14
D-glyceraldehyde 3-phosphate
Sulfolobus tokodaii
Q976X5
pH 8, 80C
147
0.09 - 0.36
Isovaleraldehyde
2200
1.8 - 158.3
NAD+
7
0.98
NADP+
Sulfolobus tokodaii
Q976X5
pH 8, 80C
10
20.67 - 107.5
propionaldehyde
273
56.66 - 257
Valeraldehyde
1481
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.9
acetaldehyde
-
-
0.19 - 0.2
Chloral hydrate
0.012 - 0.069
Disulfiram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.07
-
cell free extract
0.212
-
activity with NADP+
0.35
-
isoenzyme F1
0.378
-
activity with NAD+
0.42
-
activity in isolated mitochondria
0.49
-
after the Ni-NTA purification step
1
-
isoenzyme F2
1.48
-
recombinant enzyme, using 3-hydroxypropionaldehyde and NAD+ as substrates, at pH 8.0 and 37C
5.51
-
-
16.67
-
enzyme from breast adenocarcinoma cell line
18.92
-
-
32.95
-
enzyme from stomach mucosa
110.5
-
liver enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
benzaldehyde oxidation, enzyme from breast adenocarcinoma cell line
8 - 9
-
benzaldehyde oxidation, enzyme from stomach mucosa
9 - 9.5
-
-
9.3
-
reaction with propanal and NAD+ or NADP+
10
-
activity with NAD+, broad plateau from pH 5 to 8 followed by a sharp increase in activity to pH 10
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
pH 6.0: about 60% of maximal activity, pH 9.0: about 90% of maximal activity
6.2 - 6.7
-
pH 6.2: about 80% of maximal activity, pH 6.7: about 80% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
-
; activity assay
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 90
70C: about 50% of maximal activity, 90C: about 90% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
exhibiting oxazaphosphorine-specific acquired resistance, enzyme form ALDH3
Manually annotated by BRENDA team
-
isoform ALDH3A1
Manually annotated by BRENDA team
isozyme Aldh1a3 mRNA expression is highest in placenta
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
addition of ALDH to RASM cell homogenates significantly decreases NO consumption
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
2 * 40000, enzyme from breast adenocarcinoma cell line, SDS-PAGE
46100
-
molecular weight of one subunit, determined by gel filtration on a Superdex column and by SDS-PAGE; SDS-PAGE, recombinant enzyme
49500
-
2 * 49500, MALDI-MS
50000
4 * 50000, SDS-PAGE
51000
-
2 * 51000, SDS-PAGE
51300
4 * 51300, calculation from sequence
52682
-
4 * 52682, calculated from amino acid sequence
53904
-
x * 53904, enzyme form ALDH5, mass spectrometric analysis
54000
4 * 54000, SDS-PAGE
54570
-
x * 54570, enzyme form ALDH1, mass spectrometric analysis
55000
-
4 * 55000, SDS-PAGE
56000
2 * 56000, SDS-PAGE
57000
-
4 * 57000, SDS-PAGE
59000
-
2 * 59000, SDS-PAGE
60000
-
4 * 60000, SDS-PAGE
61000
-
4 * 61000, SDS-PAGE
100000
108000
-
enzyme from stomach mucosa, gel filtration
110000
125000
-
enzyme from breast adenocarcinoma cell line, gel filtration
170000
-
gel filtration
190000
-
190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer
200000
gel filtration
207000
-
sucrose density gradient centrifugation
220000
-
gel filtration
230000
-
isoenzyme F1, non-denaturing PAGE
240000
250000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 53904, enzyme form ALDH5, mass spectrometric analysis; x * 54570, enzyme form ALDH1, mass spectrometric analysis
homodimer
-
isoform ALDH3A1
homotetramer
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.15 M DL-malic acid pH 7.0, 18% (w/v) PEG 3350
the apoenzyme is crystallized by using 100 mM ACES, pH 6.4, 100-200 mM guanidine-HCl, 1-10 mM MgCl2 and 1617% (w/v) PEG 6000, the mutant T244A in complex with NAD is crystallized by using 100 mM ACES, pH 6.4, 100 mM guanidineHCl, 10 mM MgCl2 and 18% (w/v) PEG 6000
-
ALDHC in complex with NADPH bound in the cofactor binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel, to 1.6 A resolution. Belongs to space group P1. The ALDHC monomer comprises three distinct domains, an N-terminal cofactor (NAD/P+)-binding domain, a catalytic domain, and an oligomerization domain
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
-
pH 6.0 attenuates more than 39% of enzymatic activity. At pH 5.0, 3% of the activity remains. The enzymatic activity is completely abolished at pH values of or below 4.0
725948
5.5 - 10