Information on EC 1.2.1.18 - malonate-semialdehyde dehydrogenase (acetylating)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.2.1.18
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RECOMMENDED NAME
GeneOntology No.
malonate-semialdehyde dehydrogenase (acetylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acrylate degradation
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acetyl CoA biosynthesis
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beta-Alanine metabolism
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Inositol phosphate metabolism
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Propanoate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
3-oxopropanoate:NAD(P)+ oxidoreductase (decarboxylating, CoA-acetylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-97-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
PAO1
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-3-oxopropanoate + CoA + NAD+
propanoyl-CoA + CO2 + NADH
show the reaction diagram
-
both stereoisomers
-
?
3-oxopropanoate + CoA + NAD+
acetyl-CoA + CO2 + NADH
show the reaction diagram
3-oxopropanoate + CoA + NADP+
acetyl-CoA + CO2 + NADPH
show the reaction diagram
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
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-
-
?
malonyl-CoA + NADPH
malonate-semialdehyde + CoA + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-oxopropanoate + CoA + NAD+
acetyl-CoA + CO2 + NADH
show the reaction diagram
malonyl-CoA + NADPH
malonate-semialdehyde + CoA + NADP+
show the reaction diagram
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enzyme is involved in the autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
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cofactor; no activity with NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Disulfiram
0.01 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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0.1 M required for maximal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0053
2-Methyl-3-oxopropanoate
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-
0.0045 - 0.05
3-Oxopropanoate
0.03 - 0.1
CoA
0.15 - 0.2
NAD+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
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activity with NADP+
8.8
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activity with NAD+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.7
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pH 6.0: about 35% of maximal activity, pH 7.7: about 65% of maximal activity, activity with NADP+
7 - 9
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about 65% of maximal activity at pH 7.0 and at pH 9.0
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 58000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against phosphate buffer, pH 7.0, for 4 h at 4°C results in 80% loss of activity
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freezing and thawing causes total loss of activity even in the presence of 0.01 M 2-mercaptoethanol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, pH 7.0, in presence of 2-mercaptoethanol, stored with least loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (188 entries)
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