Information on EC 1.2.1.17 - glyoxylate dehydrogenase (acylating)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

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EC NUMBER
COMMENTARY hide
1.2.1.17
-
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RECOMMENDED NAME
GeneOntology No.
glyoxylate dehydrogenase (acylating)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glyoxylate + CoA + NADP+ = oxalyl-CoA + NADPH + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
oxalate degradation III
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Glyoxylate and dicarboxylate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
glyoxylate:NADP+ oxidoreductase (CoA-oxalylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-96-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
mango
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + CoA + NADP+
oxalyl-CoA + NADPH
show the reaction diagram
glyoxylate + CoA + NADP+
oxalyl-CoA + NADPH + H+
show the reaction diagram
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-
-
-
r
additional information
?
-
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modified TCA cycle with diminished efficacy in NADH production and decreased CO2-evolving capacity, orchestrates the synthesis of oxalate, NADPH and ATP, ingredients pivotal to the survival in an aluminium environment
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + CoA + NADP+
oxalyl-CoA + NADPH
show the reaction diagram
glyoxylate + CoA + NADP+
oxalyl-CoA + NADPH + H+
show the reaction diagram
-
-
-
-
r
additional information
?
-
-
modified TCA cycle with diminished efficacy in NADH production and decreased CO2-evolving capacity, orchestrates the synthesis of oxalate, NADPH and ATP, ingredients pivotal to the survival in an aluminium environment
-
-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Al3+
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modulation of AGODH activity, exposure of the Al-challenged cells to a control medium results in a dramatic decrease in the activity of AGODH, in contrast, control cells incubated in an Al-enriched medium display an intense activity band, AGODH may be pivotal to the adaptation of Pseudomonas fluorescens to aluminium toxicity
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-methionine
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.015
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Pseudomonas AM1 var. 470, grown on oxalate
0.024
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Pseudomonas MOx, grown on oxalate
0.144
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grown on oxalate
0.214
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Pseudomonas KOx, grown on oxalate
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
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glyoxylate + CoA + NADP+
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, several weeks
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0°C, 70% loss of activity, 24 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose
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LINKS TO OTHER DATABASES (specific for EC-Number 1.2.1.17)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)