Information on EC 1.18.1.4 - rubredoxin-NAD(P)+ reductase

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The expected taxonomic range for this enzyme is: Pyrococcus furiosus

EC NUMBER
COMMENTARY hide
1.18.1.4
-
RECOMMENDED NAME
GeneOntology No.
rubredoxin-NAD(P)+ reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 reduced rubredoxin + NAD(P)+ + H+ = 2 oxidized rubredoxin + NAD(P)H
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fatty acid degradation
-
-
non-pathway related
-
-
SYSTEMATIC NAME
IUBMB Comments
rubredoxin:NAD(P)+ oxidoreductase
The enzyme from Pyrococcus furiosus requires FAD. It reduces a number of electron carriers, including benzyl viologen, menadione and 2,6-dichloroindophenol, but rubredoxin is the most efficient. Ferredoxin is not utilized.
CAS REGISTRY NUMBER
COMMENTARY hide
114514-31-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
cytochrome c from horse heart
-
-
?
2 oxidized rubredoxin + NADH
2 reduced rubredoxin + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 oxidized rubredoxin + NADPH
2 reduced rubredoxin + NADP+ + H+
show the reaction diagram
2,6-dichloroindophenol + NADH
reduced 2,6-dichloroindophenol + NAD+ + H+
show the reaction diagram
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADH
?
show the reaction diagram
-
-
-
-
?
benzyl viologen + NADH
reduced benzyl viologen + NAD+
show the reaction diagram
-
-
-
-
?
FAD + NADH
FADH2 + NAD+
show the reaction diagram
-
-
-
-
?
Fe(III) citrate + NADH
Fe(II) citrate + NAD+
show the reaction diagram
-
-
-
-
?
FMN + NADH
FMNH2 + NAD+
show the reaction diagram
-
-
-
-
?
methyl viologen + NADH
reduced methyl viologen + NAD+ + H+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADH
reduced benzyl viologen + NAD+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADH
reduced benzyl viologen + NAD+ + H+
show the reaction diagram
-
-
-
?
oxidized benzyl viologen + NADPH
reduced benzyl viologen + NADP+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADPH
reduced benzyl viologen + NADP+ + H+
show the reaction diagram
-
-
-
?
oxidized rubredoxin + NADH
reduced rubredoxin + NAD+
show the reaction diagram
-
rubredoxin is the best substrate
-
-
?
oxidized rubredoxin + NADPH
reduced rubredoxin + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme does not reduce Pyrococcus furiosus ferredoxin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 oxidized rubredoxin + NADH
2 reduced rubredoxin + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 oxidized rubredoxin + NADPH
2 reduced rubredoxin + NADP+ + H+
show the reaction diagram
oxidized rubredoxin + NADPH
reduced rubredoxin + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
preferred physiological electron donor
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metals can be detected
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
the enzyme contains one flavin adenine dinucleotide (FAD) molecule per mol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034 - 0.18
NADH
0.005 - 0.048
NADPH
5.5
Oxidized benzyl viologen
pH 8, 80C, cosubstrate: NADH
0.0016 - 0.05
oxidized rubredoxin
-
0.0095 - 0.05
rubredoxin
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
354 - 498
NADH
258 - 341
NADPH
1236
Oxidized benzyl viologen
Pyrococcus furiosus
Q8U1K9
pH 8, 80C, cosubstrate: NADH
221 - 15000
oxidized rubredoxin
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2770 - 10400
NADH
8
5375 - 68300
NADPH
5
225
Oxidized benzyl viologen
Pyrococcus furiosus
Q8U1K9
pH 8, 80C, cosubstrate: NADH
571
22050 - 300000
oxidized rubredoxin
1837
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
pH 8.0, 80C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 80
-
about 70fold increase in kcat/Km for oxidized rebredoxin increases from 25C to 80C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39900
x * 39900, calculated from sequence
40000
x * 40000, SDS-PAGE
51000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 39900, calculated from sequence; x * 40000, SDS-PAGE
monomer
-
1 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
12 h, 50% loss of activity
95
-
2 h, 50% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli