Information on EC 1.17.5.2 - caffeine dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.17.5.2
-
RECOMMENDED NAME
GeneOntology No.
caffeine dehydrogenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
caffeine + ubiquinone + H2O = 1,3,7-trimethylurate + ubiquinol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
caffeine degradation V (bacteria, via trimethylurate)
-
-
SYSTEMATIC NAME
IUBMB Comments
caffeine:ubiquinone oxidoreductase
This enzyme, characterized from the soil bacterium Pseudomonas sp. CBB1, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-8 of caffeine. The enzyme utilizes short-tail ubiquinones as the preferred electron acceptor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CBB1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeine + ubiquinone-0
trimethyl uric acid + ubiquinol-0
show the reaction diagram
theobromine + ubiquinone-0
? + ubiquinol-0
show the reaction diagram
theophylline + ubiquinone-0
? + ubiquinol-0
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ubiquinone-0
-
preferred electron acceptor
additional information
-
enzyme is not NAD(P)+-dependent
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037
Caffeine
-
pH 7.5, 35C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167
Caffeine
Pseudomonas sp.
-
pH 7.5, 35C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1530
-
pH 7.5, 35C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
52% of maximum activity
8
-
47% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66
-
or above, activity increases linearly from 25 to 66C, which is the highest temperature allowed by the experimental conditions
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gamma subunit
32000
-
beta subunit
90000
-
alpha subunit
158000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
-
30 min, 77% residual activity
70
-
30 min, 11% residual activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 5 mM potassium phosphate buffer pH 7.5, enzyme loses activity after a few days
-