Information on EC 1.17.3.3 - 6-hydroxynicotinate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.17.3.3
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RECOMMENDED NAME
GeneOntology No.
6-hydroxynicotinate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-hydroxynicotinate + H2O + O2 = 2,6-dihydroxynicotinate + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
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-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Nicotinate and nicotinamide metabolism
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-
nicotinate degradation II
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-
SYSTEMATIC NAME
IUBMB Comments
6-hydroxynicotinate:O2 oxidoreductase
Contains [2Fe-2S] iron-sulfur centres, FAD and molybdenum. It also has a catalytically essential, labile selenium that can be removed by reaction with cyanide. In Bacillus niacini, this enzyme is required for growth on nicotinic acid.
CAS REGISTRY NUMBER
COMMENTARY hide
122191-32-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
DSM 2923
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-hydroxynicotinate + H2O + 2,6-dichlorophenol indophenol
2,6-dihydroxynicotinate + H2O2 + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
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highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor
-
?
6-hydroxynicotinate + H2O + methylene blue
2,6-dihydroxynicotinate + H2O2 + reduced methylene blue
show the reaction diagram
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highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 40% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol
-
?
6-hydroxynicotinate + H2O + nitroblue tetrazolium chloride
2,6-dihydroxynicotinate + H2O2 + reduced nitroblue tetrazolium chloride
show the reaction diagram
-
highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol
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?
6-hydroxynicotinate + H2O + O2
1,4,5,6-tetrahydro-6-oxonicotinate + H2O2
show the reaction diagram
-
-
-
-
?
6-hydroxynicotinate + H2O + O2
2,6-dihydroxynicotinate + H2O2
show the reaction diagram
6-hydroxynicotinate + H2O + phenazine ethosulfate
2,6-dihydroxynicotinate + H2O2 + reduced phenazine ethosulfate
show the reaction diagram
-
highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor
-
?
6-hydroxynicotinate + H2O + thiazolyl blue
2,6-dihydroxynicotinate + H2O2 + reduced thiazolyl blue
show the reaction diagram
-
highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol
-
?
6-hydroxynicotinate + H2O + thionin
2,6-dihydroxynicotinate + H2O2 + reduced thionin
show the reaction diagram
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highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 2% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Bactopterin
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component of the molybdenum cofactor
flavin
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covalently bound flavin
iron-sulfur centre
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contains iron-sulfur centres, iron and acid-labile sulfur
molybdenum cofactor
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur
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contains acid-labile sulfur, iron-sulfur centres
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
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at a final concentration 10% (v/v), inactivates to 30% of the initial activity
Na2S
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partially purified 6-HDH, 90% loss of activity
additional information
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not inhibited by NaCl
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
6-Hydroxynicotinate
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pH 7.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.7
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pH 7.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
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x * 85000 + x * 34000 + x * 15000, SDS-PAGE
34000
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x * 85000 + x * 34000 + x * 15000, SDS-PAGE
53000
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x * 53000, SDS-PAGE
85000
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x * 85000 + x * 34000 + x * 15000, SDS-PAGE
100000 - 140000
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non-denaturing PAGE, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
significant loss of activity during the purification procedure, lyophilization results in a complete loss of activity
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost with a half-life of 90 min in air-saturated solutions
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670692
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
free of nicotinate dehydrogenase
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purification under strictly unaerobic conditions
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