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Enzyme Nomenclature
Information on EC 1.17.2.1 - nicotinate dehydrogenase (cytochrome)
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The expected taxonomic range for this enzyme is: Pseudomonas putida
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EC NUMBER 
COMMENTARY 
1.17.2.1
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RECOMMENDED NAME
GeneOntology No.
nicotinate dehydrogenase (cytochrome)
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-cyanopyridine + 5-methylphenazinium methyl sulfate + H2O
?
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4% of the activity with nicotinate
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?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
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-
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?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinic acid + ?
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?
nipecotic acid + 5-methylphenazinium methyl sulfate + H2O
?
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8% of the activity with nicotinate
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?
picolinic acid + 5-methylphenazinium methyl sulfate + H2O
?
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7% of the activity with nicotinate
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?
pyrazine-2-carboxylic acid + 5-methylphenazinium methyl sulfate + H2O
?
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8% of the activity with nicotinate
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-
?
pyridine-3-carboxyaldehyde + 5-methylphenazinium methyl sulfate + H2O
?
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5% of the activity with nicotinate
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-
?
pyridine-3-sulfonic acid + 5-methylphenazinium methyl sulfate + H2O
?
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4% of the activity with nicotinate
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
16685
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
127874
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
130000
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE; x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
?
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE; x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c; subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c; subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
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small subunit NicA harbors the 41-C-X4-C-G-X-C-Xn-C-59 and 100-C-G-X-C-X31-C-X-C-137 conserved motifs likely involved in binding of the two [2Fe-2S] clusters; the N-terminal region of large subunit NicB contains the active site and the molybdopterin cytosine dinucleotide binding sites in an arrangement MPT2-MPT1-MPT3. The C-terminal region of NicB, residues750-1187, contains three conserved cytochrome c hemebinding motifs 817-CAVCH-823, 963-CTACH-969, and 1087-CLGCH-1093
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of gene cluster ndhSL in Pseudomonas entomophila; expression of gene cluster ndhSL in Pseudomonas entomophila
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LINKS TO OTHER DATABASES (specific for EC-Number 1.17.2.1)
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