Information on EC 1.17.2.1 - nicotinate dehydrogenase (cytochrome)

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.17.2.1
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RECOMMENDED NAME
GeneOntology No.
nicotinate dehydrogenase (cytochrome)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Nicotinate and nicotinamide metabolism
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nicotinate degradation I
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SYSTEMATIC NAME
IUBMB Comments
nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-cyanopyridine + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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4% of the activity with nicotinate
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?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
show the reaction diagram
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?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinic acid + ?
show the reaction diagram
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?
nipecotic acid + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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8% of the activity with nicotinate
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?
picolinic acid + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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7% of the activity with nicotinate
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?
pyrazine-2-carboxylic acid + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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8% of the activity with nicotinate
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?
pyridine-3-carboxyaldehyde + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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5% of the activity with nicotinate
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?
pyridine-3-sulfonic acid + 5-methylphenazinium methyl sulfate + H2O
?
show the reaction diagram
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4% of the activity with nicotinate
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2Fe-2S-center
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cytochrome c
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molybdopterin cytosine dinucleotide
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Molybdenum
subunit NdhL contains a molybdenum cofactor
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12800
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x * 23800, small subunit NicA, + x * 12800, large subunit NicB
16685
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
23800
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x * 23800, small subunit NicA, + x * 12800, large subunit NicB
127874
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
130000
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE; x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of gene cluster ndhSL in Pseudomonas entomophila; expression of gene cluster ndhSL in Pseudomonas entomophila
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