HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
Information on EC 1.17.2.1 - nicotinate dehydrogenase (cytochrome)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Pseudomonas putida
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER 
COMMENTARY 
1.17.2.1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RECOMMENDED NAME
GeneOntology No.
nicotinate dehydrogenase (cytochrome)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-cyanopyridine + 5-methylphenazinium methyl sulfate + H2O
?
-
4% of the activity with nicotinate
-
-
?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
-
-
-
?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinic acid + ?
-
-
-
-
?
nipecotic acid + 5-methylphenazinium methyl sulfate + H2O
?
-
8% of the activity with nicotinate
-
-
?
picolinic acid + 5-methylphenazinium methyl sulfate + H2O
?
-
7% of the activity with nicotinate
-
-
?
pyrazine-2-carboxylic acid + 5-methylphenazinium methyl sulfate + H2O
?
-
8% of the activity with nicotinate
-
-
?
pyridine-3-carboxyaldehyde + 5-methylphenazinium methyl sulfate + H2O
?
-
5% of the activity with nicotinate
-
-
?
pyridine-3-sulfonic acid + 5-methylphenazinium methyl sulfate + H2O
?
-
4% of the activity with nicotinate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
16685
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
127874
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
130000
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE; x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
?
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE; x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c; subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c; subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
-
small subunit NicA harbors the 41-C-X4-C-G-X-C-Xn-C-59 and 100-C-G-X-C-X31-C-X-C-137 conserved motifs likely involved in binding of the two [2Fe-2S] clusters; the N-terminal region of large subunit NicB contains the active site and the molybdopterin cytosine dinucleotide binding sites in an arrangement MPT2-MPT1-MPT3. The C-terminal region of NicB, residues750-1187, contains three conserved cytochrome c hemebinding motifs 817-CAVCH-823, 963-CTACH-969, and 1087-CLGCH-1093
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of gene cluster ndhSL in Pseudomonas entomophila; expression of gene cluster ndhSL in Pseudomonas entomophila
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LINKS TO OTHER DATABASES (specific for EC-Number 1.17.2.1)
html completed
Use of this online version of BRENDA is free for academic research only. Commercial use or download access requires a license. See terms of use.
Information
