Information on EC 1.16.8.1 - cob(II)yrinic acid a,c-diamide reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Pseudomonas denitrificans

EC NUMBER
COMMENTARY hide
1.16.8.1
-
RECOMMENDED NAME
GeneOntology No.
cob(II)yrinic acid a,c-diamide reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 cob(I)yrinic acid a,c-diamide + FMN + 2 H+ = 2 cob(II)yrinic acid a,c-diamide + FMNH2
show the reaction diagram
; This enzyme also catalyses the reduction of cob(II)yric acid, cob(II)inamide, cob(II)inamide phosphate, GDP-cob(II)inamide and cob(II)alamin although cob(II)yrinic acid a,c-diamide is thought to be the physiological substrate. Also uses FAD and NADH but not NADPH
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenosylcobalamin biosynthesis from cobyrinate a,c-diamide I
-
-
adenosylcobalamin biosynthesis from cobyrinate a,c-diamide II
-
-
vitamin B12 metabolism
-
-
Porphyrin and chlorophyll metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
cob(I)yrinic acid-a,c-diamide:FMN oxidoreductase
This enzyme also catalyses the reduction of cob(II)yric acid, cob(II)inamide, cob(II)inamide phosphate, GDP-cob(II)inamide and cob(II)alamin although cob(II)yrinic acid a,c-diamide is thought to be the physiological substrate [1]. Also uses FAD and NADH but not NADPH.
CAS REGISTRY NUMBER
COMMENTARY hide
145539-93-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 cob(II)yrinic acid a,c-diamide + FMN
cob(I)yrinic acid a,c-diamide + FMNH2
show the reaction diagram
-
-
-
-
?
cob(II)alamin + FMN
?
show the reaction diagram
-
41% activity compared to cob(II)yric acid
-
?
cob(II)inamide + FMN
?
show the reaction diagram
-
67-48% activity compared to cob(II)yric acid, percentage depends on method of activity detection
-
?
cob(II)inamide phosphate + FMN
?
show the reaction diagram
-
66% activity compared to cob(II)yric acid
-
?
cob(II)yriC acid + FMN
?
show the reaction diagram
-
-
-
?
cob(II)yrinic acid a,c-diamide + FMN
?
show the reaction diagram
-
-
-
?
cob(II)yrinic acid a,c-diamide + FMN
cob(I)yrinic acid a,c-diamide + FMNH2
show the reaction diagram
GDP-cob(II)inamide + FMN
?
show the reaction diagram
-
71% activity compared to cob(II)yric acid
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 cob(II)yrinic acid a,c-diamide + FMN
cob(I)yrinic acid a,c-diamide + FMNH2
show the reaction diagram
-
-
-
-
?
cob(II)yrinic acid a,c-diamide + FMN
?
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0059
FMN
-
0.2 M Tris-HCl buffer, pH 8.0, 30°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
gel permeation HPLC
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 16000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6,300-fold purification including Mono Q and FMN agarose chromatography
-
Show AA Sequence (251 entries)
Please use the Sequence Search for a certain query.