Information on EC 1.16.1.5 - aquacobalamin reductase (NADPH)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.16.1.5
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RECOMMENDED NAME
GeneOntology No.
aquacobalamin reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 cob(II)alamin + NADP+ + 2 H2O = 2 aquacob(III)alamin + NADPH + H+
show the reaction diagram
A flavoprotein; A flavoprotein. Acts on aquacob(III)alamin and hydroxycobalamin, but not on cyanocobalamin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Porphyrin and chlorophyll metabolism
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SYSTEMATIC NAME
IUBMB Comments
cob(II)alamin:NADP+ oxidoreductase
A flavoprotein. Acts on aquacob(III)alamin and hydroxycobalamin, but not on cyanocobalamin.
CAS REGISTRY NUMBER
COMMENTARY hide
110777-32-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
freshwater fish
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Manually annotated by BRENDA team
strain SM-ZK
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
sea fish
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Manually annotated by BRENDA team
monkey
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Manually annotated by BRENDA team
frog
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADPH
2 ferrocyanide + NADP+ + H+
show the reaction diagram
aquacob(III)alamin + NADPH + H+
cob(II)alamin + NADP+
show the reaction diagram
-
reaction leads to stimulation of the conversion of apomethionine synthase and aquacobalamin to the methionine synthase holoenzyme
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-
?
NADPH + aquacob(III)alamin
NADP+ + cob(II)alamin
show the reaction diagram
NADPH + cytochrome c
?
show the reaction diagram
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
NADPH + hydroxycobalamin
NADP+ + 5'-deoxyadenosylcobalamin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aquacob(III)alamin + NADPH + H+
cob(II)alamin + NADP+
show the reaction diagram
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reaction leads to stimulation of the conversion of apomethionine synthase and aquacobalamin to the methionine synthase holoenzyme
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?
NADPH + aquacob(III)alamin
NADP+ + cob(II)alamin
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
Ba2+
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mitochondrial enzyme
Ca2+
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mitochondrial enzyme
Co2+
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mitochondrial enzyme
Cu2+
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complete inhibition at 1 mM, mitochondrial enzyme
Fe2+
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mitochondrial enzyme
Hg2+
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complete inhibition at 1 mM, mitochondrial enzyme
Mersalyl
Mg2+
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mitochondrial enzyme
Mn2+
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mitochondrial enzyme
N-ethylmaleimide
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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slightly activating at 1 mM, mitochondrial enzyme
o-phenanthroline
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slightly activating at 1 mM, mitochondrial enzyme
Phenobarbital
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mitochondrial enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.058 - 0.208
aquacobalamin
0.055
hydroxycobalamin
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0.0048 - 0.043
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0045
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mitochondrial, mutant cbl C
0.0046
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mitochondrial, wild-type
0.0064
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microsomal, mutant cbl A
0.0069
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microsomal, mutant cbl C
0.0083
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microsomal, wild-type
0.0152
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mitochondrial enzyme, crude extract
0.023
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microsomal crude extract
0.024
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mitochondrial crude extract
0.0365
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mitochondrial crude extract from phenobarbital-treated rats
0.0807
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microsomal crude extract from phenobarbital-treated rats
2.24
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purified enzyme from mitochondria
2.9
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substrate aquacobalamin, purified mitochondrial enzyme, aerobic conditions
4.9
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purified mitochondrial enzyme, substrate potassium ferricyanide
6.4
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purified mitochondrial enzyme, substrate aquacobalamin
11.22
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purified microsomal enzyme
14.2
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purified mitochondrial enzyme, substrate 2,6-dichlorophenolindophenol, aerobic conditions
15.4
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purified mitochondrial enzyme, substrate cytochrome c
16.8
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purified mitochondrial enzyme, substrate 2,6-dichlorophenolindophenol
25
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purified mitochondrial enzyme, substrate potassium ferricyanide, aerobic conditions
37
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purified mitochondrial enzyme, substrate cytochrome c, aerobic conditions
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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microsomal enzyme
8.2
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mitochondrial enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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microsomal enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
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gel filtration, SDS-PAGE
79000
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microsomal enzyme, gel filtration
additional information
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MW 166000 Da of native mitochondrial enzyme as part of pyruvate:NADP+ oxidoreductase, immunoblotting
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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below, complete loss of activity
438044
6 - 8
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10 min, 55°C, stable
438041, 438044
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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10 min, pH 7.0, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 to 80°C, 10 mM Tris-acetate, pH 7.0, potassium chloride, 1 mM EDTA, 0.001 mM dithiothreitol, concentrated, no loss of activity in several months
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0°C, 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from microsomes
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from mitochondria and microsomes
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mitochondrial enzyme
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mitochondrial enzyme; pale yellow coloured
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mitochondrial, light yellow coloured
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partially
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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mitochondrial enzyme mutants cbl C and cbl A, cbl C mutant is unchanged compared to wild-type, while cbl A mutant shows no NADPH-linked activity