Information on EC 1.16.1.4 - cob(II)alamin reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.16.1.4
-
RECOMMENDED NAME
GeneOntology No.
cob(II)alamin reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 cob(I)alamin + NAD+ = 2 cob(II)alamin + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Porphyrin and chlorophyll metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
cob(I)alamin:NAD+ oxidoreductase
A flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-40-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NADH + cob(II)alamine
NAD+ + cob(I)alamin
show the reaction diagram
additional information
?
-
-
enzyme specifically interacts with PduO ATP:cob(I)alamin adenosyltransferase and cob(I)alamin is sequestered during the conversion of cob(II)alamin by these two enzymes
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADH + cob(II)alamine
NAD+ + cob(I)alamin
show the reaction diagram
additional information
?
-
-
enzyme specifically interacts with PduO ATP:cob(I)alamin adenosyltransferase and cob(I)alamin is sequestered during the conversion of cob(II)alamin by these two enzymes
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
flavoprotein, stimulated equally well by FAD or FMN
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00022
-
FAD repalced by FMN
0.00032
-
with dithiothreitol as electron donor
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
x * 48400, calculated, x * 48000, SDS-PAGE
48400
-
x * 48400, calculated, x * 48000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 48400, calculated, x * 48000, SDS-PAGE
additional information
-
enzyme specifically interacts with PduO ATP:cob(I)alamin adenosyltransferase and cob(I)alamin is sequestered during the conversion of cob(II)alamin by these two enzymes
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
under N2-atmosphere, cell-free extract, several days
50
-
5 min, about 40% loss of activity
60
-
5 min, 75% loss of activity
70
-
5 min, about 90% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE