Information on EC 1.14.99.45 - carotene epsilon-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.99.45
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RECOMMENDED NAME
GeneOntology No.
carotene epsilon-monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-carotene + AH2 + O2 = alpha-cryptoxanthin + A + H2O
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
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epsilon-ring hydroxylation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lutein biosynthesis
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carotenoid biosynthesis
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Carotenoid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
alpha-carotene:oxygen oxidoreductase (3-hydroxylating)
A heme-thiolate protein (P-450). Also acts on zeinoxanthin to give lutein.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mRNA; cv. Zhongshu 4, gene LUT1
BT012891
GenBank
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-carotene + AH2 + O2
alpha-cryptoxanthin + A + H2O
show the reaction diagram
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CYP97C1 has alpha-carotene epsilon-ring hydroxylase activity
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-
?
alpha-carotene + O2 + AH2
alpha-cryptoxanthin + A + H2O
show the reaction diagram
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-
-
-
?
alpha-carotene + reduced cytochrome P40 + O2
lutein + oxidized cytochrome P40 + H2O
show the reaction diagram
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-
-
?
beta-carotene + reduced cytochrome P40 + O2
zeaxanthin + oxidized cytochrome P40 + H2O
show the reaction diagram
-
-
-
?
zeinoxanthin + AH2 + O2
lutein + A + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-carotene + reduced cytochrome P40 + O2
lutein + oxidized cytochrome P40 + H2O
show the reaction diagram
Q6TBX7, Q93VK5
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-
-
?
beta-carotene + reduced cytochrome P40 + O2
zeaxanthin + oxidized cytochrome P40 + H2O
show the reaction diagram
Q6TBX7, Q93VK5
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-
-
?
zeinoxanthin + AH2 + O2
lutein + A + H2O
show the reaction diagram
BT012891
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-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
a heme-containing cytochrome P450 enzyme; a heme-containing cytochrome P450 enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene LeLUT1, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, expression of gene LeLUT1-GFP fusion protein in chloroplasts of Arabidopsis thaliana mesophyll protoplasts, quantitative real-time PCR expression analysis
BT012891
genes lut1 and lut5, real-time PCR expression analysis; genes lut1 and lut5, real-time PCR expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of LeLUT1 is downregulated by various abiotic stresses in tomato
BT012891
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information