Information on EC 1.14.99.38 - cholesterol 25-hydroxylase

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The expected taxonomic range for this enzyme is: Tetrapoda

EC NUMBER
COMMENTARY hide
1.14.99.38
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RECOMMENDED NAME
GeneOntology No.
cholesterol 25-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
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reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Primary bile acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs).
CAS REGISTRY NUMBER
COMMENTARY hide
60202-07-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
gene Ch25h
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
desmosterol
troleandomycin
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25-hydroxycholesterol
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Kdo2-lipid A
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lipopolysaccharide
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additional information
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incubation with LPS derived from Escherichia coli or Salmonella enterica induces CH25H mRNA
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.73
sequence calculation
6.75
sequence calculation
6.77
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sequence calculation
6.88
sequence calculation
7.07
sequence calculation
7.67
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sequence calculation
8.15
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sequence calculation
8.61
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sequence calculation
8.88
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sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30426
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x * 30426, sequence calculation
31326
x * 31326, sequence calculation
31405
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x * 31405, sequence calculation
31464
x * 31464, sequence calculation
31700
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x * 31700
31745
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x * 31745, sequence calculation
31850
x * 31850, sequence calculation
32456
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x * 32456, sequence calculation
33000
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determined by SDS-PAGE and Western Blot analysis
34414
x * 34414, sequence calculation
34672
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x * 34672, sequence calculation
34700
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x * 34700
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
gene CH25H, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
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gene CH25H, overexpression in HEK-293T cells
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gene Ch25h, quantitative real-time RT-PCR expression analysis
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intronless gene CH25H is located on chromosome 1, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 10, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
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intronless gene CH25H is located on chromosome 19, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
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intronless gene CH25H is located on chromosome 26, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 6, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
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intronless gene CH25H is located on chromosome 9, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection
the gene encoding the enzyme is located on chromosome 10
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activation of the innate immune system by TLR ligands induces 25-hydroxycholesterol production which results in suppression of IgA class switching in B cells
both IFN-alpha- and IFN-gamma induce the enzyme in bone marrow-derived macrophages
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induction of Ch25h is JAK- and STAT1-dependent, overview
induction of CYP3A by pregnenolone-16 alpha-carbonitrile causes accumulation of 25-hydroxycholesterol in cell line AML12 derived from mouse liver, the induction is not suppressed by the addition of desmosterol. Although the transcription of Ch25h is also upregulated by addition of pregnenolone-16 alpha-carbonitrile, the protein level of CH25H is not elevated. Desmosterol does not affect the expression of cellular CYP3A protein, but CH25H protein level is obviously decreased by desmosterol treatment
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine