Information on EC 1.14.99.26 - 2-hydroxypyridine 5-monooxygenase

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The expected taxonomic range for this enzyme is: Brevibacillus brevis

EC NUMBER
COMMENTARY hide
1.14.99.26
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RECOMMENDED NAME
GeneOntology No.
2-hydroxypyridine 5-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-hydroxypyridine + AH2 + O2 = 2,5-dihydroxypyridine + A + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
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oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
2-hydroxypyridine,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)
Also oxidizes 2,5-dihydroxypyridine, but does not act on 3-hydroxypyridine, 4-hydroxypyridine or 2,6-dihydroxypyridine.
CAS REGISTRY NUMBER
COMMENTARY hide
96779-45-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated by enrichment on isonicotinic acid
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-hydroxypyridine + NADH + O2
2,5-dihydroxypyridine + NAD+ + H2O
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-hydroxypyridine + NADH + O2
2,5-dihydroxypyridine + NAD+ + H2O
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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2% activity of NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
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85% inhibition at 1.7 mM, 2 min preincubation
Na2As2O3
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partial inhibition
NaF
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53% inhibition at 1.7 mM, 5 min preincubation
NaN3
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53% inhibition at 1.7 mM, 5 min preincubation
NiCl2
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47% inhibition at 1.7 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 8.5
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half-maximal activity at pH 6.3 and pH 8.5
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
45 mM dithiothreitol affords partial protection against denaturation
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50% glycerol affords partial protection against denaturation
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dialysis of extracts in cold, 4C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss
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enzyme is completely blocked by anaerobic conditions
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C or 0C, overnight, complete loss of activity
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-20C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d
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