Information on EC 1.14.99.21 - Latia-luciferin monooxygenase (demethylating)

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The expected taxonomic range for this enzyme is: Latia neritoides

EC NUMBER
COMMENTARY hide
1.14.99.21
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RECOMMENDED NAME
GeneOntology No.
Latia-luciferin monooxygenase (demethylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Latia luciferin + AH2 + 2 O2 = oxidized Latia luciferin + CO2 + formate + A + H2O + hnu
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
demethylation
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oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
Latia-luciferin,hydrogen-donor:oxygen oxidoreductase (demethylating)
A flavoprotein. Latia is a bioluminescent mollusc. The reaction possibly involves two enzymes, an oxygenase followed by a monooxygenase for the actual light-emitting step.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-54-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1E)-2-methyl-4-(2,3,6-trimethylphenyl)but-1-en-1-yl formate + O2
4-(2,3,6-trimethylphenyl)butan-2-one + CO2 + formate
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,4,5-trimethylphenyl)but-1-en-1-yl formate + O2
4-(2,4,5-trimethylphenyl)butan-2-one + CO2 + formate
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,4,5-trimethylphenyl)but-1-en-1-yl formate + reduced electron acceptor + O2
4-(2,4,5-trimethylphenyl)butane-2-one + CO2 + formate + electron acceptor + H2O + hv
show the reaction diagram
-
-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl 4-(methylamino)benzoate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + 4-(methylamino)benzaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl 4-cyanobenzoate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + 4-cyanobenzaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl 4-methoxybenzoate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + 4-methoxybenzaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl 4-nitrobenzoate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + 4-nitrobenzaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl acetate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + acetaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl benzoate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + benzaldehyde
show the reaction diagram
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-
-
-
?
(1E)-2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-1-en-1-yl formate + O2
4-(2,6,6-trimethylcyclohex-1-en-1-yl)butan-2-one + CO2 + formate
show the reaction diagram
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-
-
-
?
(1E)-4-(2,6-dimethylphenyl)-2-methylbut-1-en-1-yl formate + O2
4-(2,6-dimethylphenyl)butan-2-one + CO2 + formate
show the reaction diagram
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-
-
-
?
(1E)-4-(2,6-dimethylphenyl)-2-methylbut-1-en-1-yl formate + reduced electron acceptor + O2
4-(2,6-dimethylphenyl)butane-2-one + CO2 + formate + electron acceptor + H2O + hv
show the reaction diagram
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-
-
-
?
(1E)-4-mesityl-2-methylbut-1-en-1-yl formate + reduced electron acceptor + O2
4-mesitylbutane-2-one + CO2 + formate + electron acceptor + H2O + hv
show the reaction diagram
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-
-
-
?
(E)-2-methyl-4-(2,6,6-trimethyl-1-cyclohex-1-yl)-1-buten-1-ol-formate + NADH + O2
oxidized Latia luciferin + NAD+ + CO2 + H2O + formate + light
show the reaction diagram
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trivial name Latia luciferin
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?
Latia luciferin + reduced electron acceptor + O2
oxidized Latia luciferin + CO2 + formate + electron acceptor + H2O + hv
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
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activation, especially together with optimal NADH
FMN
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flavoprotein, reductase component
FMNH2
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luciferase
NADH
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reductase component
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1E)-2-methyl-4-(2-methylphenyl)but-1-en-1-yl formate
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IC50: 0.460 mM
(1E)-2-methyl-4-phenylbut-1-en-1-yl formate
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IC50: 6.96 mM
oxyluciferin
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IC50: 0.436 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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strong increase of activity
Purple protein
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requirement
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.46
(1E)-2-methyl-4-(2-methylphenyl)but-1-en-1-yl formate
Latia neritoides
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IC50: 0.460 mM
6.96
(1E)-2-methyl-4-phenylbut-1-en-1-yl formate
Latia neritoides
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IC50: 6.96 mM
0.436
oxyluciferin
Latia neritoides
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IC50: 0.436 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
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assay at
25
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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6 * 30000, Superdex 200 gel filtration
31000
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SDS-PAGE
31600
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MALDI-TOF
180000
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Superdex 200 gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
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6 * 30000, Superdex 200 gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HiPrep Sephacryl S-200 column chromatography, HiTrap Con A chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
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