Information on EC 1.14.99.20 - phylloquinone monooxygenase (2,3-epoxidizing)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.14.99.20
-
RECOMMENDED NAME
GeneOntology No.
phylloquinone monooxygenase (2,3-epoxidizing)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phylloquinone + AH2 + O2 = 2,3-epoxyphylloquinone + A + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phylloquinone,hydrogen-donor:oxygen oxidoreductase (2,3-epoxidizing)
-
CAS REGISTRY NUMBER
COMMENTARY hide
54596-37-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fetus, neonate, 1-18 years
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phylloquinone + electron donor + O2
2,3-epoxyphylloquinone + oxidized electron + H2O
show the reaction diagram
phylloquinone + electron donor + O2
2,3-epoxyphylloquinone + oxidized electron donor + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phylloquinone + electron donor + O2
2,3-epoxyphylloquinone + oxidized electron + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-chloro-3-phytyl-1,4-naphthoquinone
-
-
glutathione peroxidase
-
competitive inhibitor
-
Tetrachloro-4-pyridinol
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutamate-containing substrates
-
bound to the vitamin K-dependent carboxylase converts its vitamin K epoxidase function from an inactive to an active state
-
Mn2+
-
activating effect is limited to peptide substrate with vicinal glutamyl residues
propeptide
-
bound to the vitamin K-dependent carboxylase converts its vitamin K epoxidase function from an inactive to an active state
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00667
Phe-Leu-Glu-Glu-Leu
Bos taurus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00077 - 0.00088
-
-
0.00466
-
-
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17
-
enzyme assay
25
-
enzyme assay
37
-
enzyme assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
77000
-
x * 77000, SDS-PAGE
94000
-
x * 94000, SDS-PAGE, wild type enzyme
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
preincubation for 15 min at 37C results in an almost complete inhibition of the epoxidation
-
stable for several days when left on ice, freezing and thawing destroys the activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, lyophilized, stable for up to 1 month
-
-80C, 20% glycerol
-
-80C, soluble enzyme preparation, at least 5% glycerol, stable to multiple freeze and thaw cycles
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in chinese hamster ovary cells CHO-Dukx-B11
-
wild-type and mutants of bovine enzyme species expressed in baculovirus-infected Spodoptera frugiperda cells
-
wild-type and mutants of bovine enzyme species expressed in chinese hamster ovary cells CHO-Dukx-B11
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H160A
-
94% of wild-type activity
H287A
-
163% of wild-type activity
H381A
-
142% of wild-type activity
K218A
-
no residual activity, but exogenous amines restore activity and its degree depends on the basicity of the amine