Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11-deoxycorticosterone + AH2 + O2
? + A + H2O
11-deoxycortisol + AH2 + O2
? + A + H2O
11alpha-hydroxyprogesterone + NADPH + O2
6beta,11alpha-dihydroxyprogesterone
16,17alpha-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16,17alpha-epoxyprogesterone + A + H2O
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
19-nortestosterone + NADPH + O2
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
additional information
?
-
11-deoxycorticosterone + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycorticosterone + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycortisol + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycortisol + AH2 + O2
? + A + H2O
-
-
-
-
?
11alpha-hydroxyprogesterone + NADPH + O2
6beta,11alpha-dihydroxyprogesterone
-
-
-
?
11alpha-hydroxyprogesterone + NADPH + O2
6beta,11alpha-dihydroxyprogesterone
-
-
-
?
16,17alpha-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16,17alpha-epoxyprogesterone + A + H2O
-
-
-
?
16,17alpha-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16,17alpha-epoxyprogesterone + A + H2O
-
-
-
?
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
-
-
-
-
?
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
19-nortestosterone + NADPH + O2
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
-
?
19-nortestosterone + NADPH + O2
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
multicomponent monooxygenase system
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
necessary step in the synthesis of cortico-steroid hormones
-
?
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
-
-
-
-
?
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
-
-
-
-
?
additional information
?
-
the enzyme shows low activity on progesterone and no activities on testosterone and 11-deoxycortisol
-
-
?
additional information
?
-
-
the enzyme shows low activity on progesterone and no activities on testosterone and 11-deoxycortisol
-
-
?
additional information
?
-
the enzyme shows low activity on progesterone and no activities on testosterone and 11-deoxycortisol
-
-
?
additional information
?
-
-
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
-
-
?
additional information
?
-
-
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
-
-
?
additional information
?
-
the enzyme is capable of converting useful pharmaceutical targets such as 3-oxo-DELTA4-steroids (e.g., testosterone, 11-deoxycorticosterone, androstenedione, and progesterone) mainly into their 15beta-hydroxy homologues. In addition, the enzyme hydroxylates progesterone to a minor extent at the 11alpha-, 9alpha-, and 6beta-positions
-
-
?
additional information
?
-
CYP106A2-catalyzed hydroxylations of progesterone at the 15beta-, 11alpha-, 9alpha-, and 6beta-positions
-
-
?
additional information
?
-
-
hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system
-
-
?
additional information
?
-
-
hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system
-
-
?
additional information
?
-
-
multi-enzyme complex contains cytochrome P450 and NADPH cytochrome c reductase
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11-deoxycorticosterone + AH2 + O2
? + A + H2O
11-deoxycortisol + AH2 + O2
? + A + H2O
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
additional information
?
-
the enzyme is capable of converting useful pharmaceutical targets such as 3-oxo-DELTA4-steroids (e.g., testosterone, 11-deoxycorticosterone, androstenedione, and progesterone) mainly into their 15beta-hydroxy homologues. In addition, the enzyme hydroxylates progesterone to a minor extent at the 11alpha-, 9alpha-, and 6beta-positions
-
-
?
11-deoxycorticosterone + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycorticosterone + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycortisol + AH2 + O2
? + A + H2O
-
-
-
-
?
11-deoxycortisol + AH2 + O2
? + A + H2O
-
-
-
-
?
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
-
-
-
-
?
16alpha,17-epoxyprogesterone + AH2 + O2
11alpha-hydroxy-16alpha,17-epoxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
17alpha-hydroxyprogesterone + AH2 + O2
11alpha,17alpha-dihydroxyprogesterone + A + H2O
-
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
-
?
progesterone + AH2 + O2
11alpha-hydroxyprogesterone + A + H2O
-
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
activity dependent upon NADPH and O2
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
-
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
multicomponent monooxygenase system
-
?
progesterone + NADPH + O2
11alpha-hydroxyprogesterone + NADP+ + H2O
-
necessary step in the synthesis of cortico-steroid hormones
-
?
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
-
-
-
-
?
testosterone + AH2 + O2
11alpha-hydroxytestosterone + A + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.02 - 0.203
progesterone
0.02
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395I
0.026
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395W/G397K
0.028
progesterone
pH not specified in the publication, 30°C, mutant D217V/A395I
0.032
progesterone
pH not specified in the publication, 30°C, mutants A395I and M155I/F165L/A395I
0.038
progesterone
pH not specified in the publication, 30°C, mutant T247V/A395I
0.039
progesterone
pH not specified in the publication, 30°C, mutant T247W/A395W/G379K
0.046
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I/R409L
0.048
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395W/G379K/R409L
0.052
progesterone
pH not specified in the publication, 30°C, mutant T248V/A395I
0.055
progesterone
pH not specified in the publication, 30°C, mutant A395W/G397K
0.084
progesterone
pH not specified in the publication, 30°C, mutant T89N/A395I
0.091
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I
0.109
progesterone
pH not specified in the publication, 30°C, mutant T89N/A106T/A395I/R409L
0.203
progesterone
pH not specified in the publication, 30°C, wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.3
progesterone
pH not specified in the publication, 30°C, wild-type
0.9
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395W/G397K
1.1
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395I
1.2
progesterone
pH not specified in the publication, 30°C, mutant A395W/G397K
1.3
progesterone
pH not specified in the publication, 30°C, mutant A395I
1.9
progesterone
pH not specified in the publication, 30°C, mutant T247V/A395I
2.5
progesterone
pH not specified in the publication, 30°C, mutant T89N/A106T/A395I/R409L
2.7
progesterone
pH not specified in the publication, 30°C, mutant T247W/A395W/G379K
2.9
progesterone
pH not specified in the publication, 30°C, mutant T89N/A395I
3.5
progesterone
pH not specified in the publication, 30°C, mutant T248V/A395I
4.3
progesterone
pH not specified in the publication, 30°C, mutant D217V/A395I
4.5
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395W/G379K/R409L
5
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I
6.4
progesterone
pH not specified in the publication, 30°C, mutant M155I/F165L/A395I
6.9
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I/R409L
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.4
progesterone
pH not specified in the publication, 30°C, wild-type
21.7
progesterone
pH not specified in the publication, 30°C, mutant A395W/G397K
23.2
progesterone
pH not specified in the publication, 30°C, mutant T89N/A106T/A395I/R409L
34.2
progesterone
pH not specified in the publication, 30°C, mutant T89N/A395I
34.5
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395W/G397K
42
progesterone
pH not specified in the publication, 30°C, mutants A395II
49.9
progesterone
pH not specified in the publication, 30°C, mutant T247V/A395I
53.1
progesterone
pH not specified in the publication, 30°C, mutant A243V/A395I
55.1
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I
66.8
progesterone
pH not specified in the publication, 30°C, mutant T248V/A395I
70.6
progesterone
pH not specified in the publication, 30°C, mutant T247W/A395W/G379K
93.4
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395W/G379K/R409L
151.4
progesterone
pH not specified in the publication, 30°C, mutant A106T/A395I/R409L
151.5
progesterone
pH not specified in the publication, 30°C, mutant D217V/A395I
201.8
progesterone
pH not specified in the publication, 30°C, mutant M155I/F165L/A395I
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A106T/A395I
site-directed mutagenesis, the mutant shows 14.3fold higher regioselectivity for 11alpha-hydroxylation and 39.3fold increased catalytic efficiency compared to the wild-type enzyme
A106T/A395I/R409L
site-directed mutagenesis, the mutant shows 12.6fold higher regioselectivity for 11alpha-hydroxylation and 108fold increased catalytic efficiency compared to the wild-type enzyme
A106T/A395W/G379K/R409L
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
A243V/A395I
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
A243V/A395W/G397K
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
A395I
site-directed mutagenesis, the mutant shows 8.9fold higher 11alpha-hydroxylation activity compared to the wild-type enzyme
A395W/G397K
site-directed mutagenesis, the mutant shows 11.5fold higher 11alpha-hydroxylation activity compared to the wild-type enzyme
D217V/A395I
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
M155I/F165L/A395I
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
T247V/A395I
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
T247W/A395W/G379K
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
T248V/A395I
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
T89N/A106T/A395I/R409L
site-directed mutagenesis, the mutant shows higher regioselectivity for 11alpha-hydroxylation and increased catalytic efficiency compared to the wild-type enzyme
T89N/A395I
site-directed mutagenesis, the mutant shows 11.8fold higher regioselectivity for 11alpha-hydroxylation and 24.4fold increased catalytic efficiency compared to the wild-type enzyme. The production of 15beta-hydroxyprogesterone decreases from 50.4% of the wild-type to 4.8% of mutant T89N/A395I enzyme, whereas that of 11alpha-hydroxyprogesterone increases from 27.7% to 80.9%
additional information
direction of the regioselectivity of the enzyme towards hydroxylation at position 11 in the C ring of the steroid through a combination of saturation mutagenesis and rational site-directed mutagenesis, with aid of data from a homology model of CYP106A2 containing docked progesterone, together with site-directed mutagenesis of active site residues. Distributions of amounts of monohydroxylated progesterone products (15beta-,11alpha-, 9alpha-, and 6beta-hydroxyprogesterone) in vivo of wild-type and mutant enzymes, overview
additional information
-
gene CYP509C12, production of a recombinant Pichia pastoris engieered to coexpress gene CYP509C12, ecoding the enzyme with the NAD(P)H-dependent reductase also from Rhizopus oryzae, to improve electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone and is useful for biotechnologic applications
additional information
-
gene CYP509C12, production of a recombinant Pichia pastoris engieered to coexpress gene CYP509C12, ecoding the enzyme with the NAD(P)H-dependent reductase also from Rhizopus oryzae, to improve electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone and is useful for biotechnologic applications
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Jayanthi, C.R.; Madyastha, P.; Madyastha, K.M.
Microsomal 11alpha-hydroxylation of progesterone in Aspergillus ochraceus: Part I: Characterization of the hydroxylase system
Biochem. Biophys. Res. Commun.
106
1262-1268
1982
Aspergillus ochraceus, Rhizopus arrhizus, Rhizopus stolonifer, Aspergillus ochraceus NRRL 405, Aspergillus ochraceus TS
brenda
Shibahara, M.; Moody, J.A.; Smith, L.L.
Microbial hydroxylations. V. 11alpha-hydroxylation of progesterone by cell-free preparations of Aspergillus ochraceus
Biochim. Biophys. Acta
202
172-179
1979
Aspergillus ochraceus, Priestia megaterium, Aspergillus ochraceus NRRL 405
brenda
Hanisch, W.H.; Dunnill, P.
Regeneration of the progesterone 11alpha-hydroxylase of Rhizopus nigricans by the use of periodate
Biotechnol. Lett.
2
123-126
1980
Rhizopus stolonifer
-
brenda
Ghosh, D.; Samanta, T.B.
11alpha-Hydroxylation of progesterone by cell free preparation of Aspergillus ochraceus TS
J. Steroid Biochem.
14
1063-1067
1981
Aspergillus ochraceus, Aspergillus ochraceus TS
brenda
Cresnar, B.; Breskvar, K.; Hudnik-Plevnik, T.
Resolution and reconstitution of the NADPH-cytochrome c (P-450) reductase induced by progesterone in Rhizopus nigricans
Biochem. Biophys. Res. Commun.
133
1057-1063
1985
Rhizopus stolonifer
brenda
Broad, D.F.; Pontin, S.; Dunnill, P.
Studies for the large scale isolation of membrane-associated progesterone 11alpha-hydroxylase:enzyme stability in Rhizopus nigricans ATCC 6277b cells
Enzyme Microb. Technol.
9
546-548
1986
Rhizopus stolonifer
brenda
Bonnerjea, J.; Pontin, S.; Hoare, M.; Dunnill, P.
Poly(ethylene glycol) precipitation and two aquaeous-phase separation of progesterone 11-alpha hydroxylase from Rhizopus nigricans
Appl. Microbiol. Biotechnol.
27
362-365
1988
Rhizopus stolonifer
brenda
Lenasi, H.; Hudnik-Plevnik, T.
Identification and partial characterization of cytosolic progesterone-binding sites in the filamentous fungus Rhizopus nigricans
Arch. Biochem. Biophys.
330
80-86
1996
Rhizopus stolonifer
brenda
Dutta, T.K.; Samanta, T.B.
Bioconversion of progesterone by the activated immobilized conidia of Aspergillus ochraceus TS
Curr. Microbiol.
39
309-312
1999
Aspergillus ochraceus, Aspergillus ochraceus G8, Aspergillus ochraceus TS, Rhizopus stolonifer
brenda
Kunic, B.; Makovec, T.; Breskvar, K.
Comparison of two monooxygenase systems with cytochrome P450 in filamentous fungus Rhizopus nigricans
Pflugers Arch.
439
R107-108
2000
Rhizopus stolonifer
brenda
Petric, S.; Hakki, T.; Bernhardt, R.; Zigon, D.; Cresnar, B.
Discovery of a steroid 11alpha-hydroxylase from Rhizopus oryzae and its biotechnological application
J. Biotechnol.
150
428-437
2010
Rhizopus arrhizus, Rhizopus arrhizus RA 99-880
brenda
Nguyen, K.T.; Virus, C.; Guennewich, N.; Hannemann, F.; Bernhardt, R.
Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone
ChemBioChem
13
1161-1166
2012
Priestia megaterium (Q06069)
brenda
Chen, X.; Luo, X.; Cao, F.; Zhu, T.; Fan, Y.; Jia, X.; Shen, Y.
Molecular cloning, expression of CPR gene from Rhizopus oryzae into Rhizopus nigericans and its application in the 11alpha-hydroxylation of 16alpha, 17-epoxy-progesterone
Enzyme Microb. Technol.
66
28-34
2014
Rhizopus arrhizus, Rhizopus arrhizus CICC 40467
brenda
Wang, R.; Sui, P.; Hou, X.; Cao, T.; Jia, L.; Lu, F.; Singh, S.; Wang, Z.; Liu, X.
Cloning and identification of a novel steroid 11alpha-hydroxylase gene from Absidia coerulea
J. Steroid Biochem. Mol. Biol.
171
254-261
2017
Absidia caerulea (A0A173GQ95), Absidia caerulea, Absidia caerulea AS3.65 (A0A173GQ95)
brenda
Gao, Q.; Qiao, Y.; Shen, Y.; Wang, M.; Wang, X.; Liu, Y.
Screening for strains with 11alpha-hydroxylase activity for 17alpha-hydroxy progesterone biotransformation
Steroids
124
67-71
2017
Aspergillus ochraceus, Beauveria bassiana, Cunninghamella elegans, Metarhizium anisopliae, Rhizopus stolonifer, Rhizopus stolonifer CGMCC33900, Aspergillus ochraceus CICC41473, Cunninghamella elegans CICC40250, Metarhizium anisopliae ACCC30194, Beauveria bassiana ACCC30728
brenda