Information on EC 1.14.21.8 - pseudobaptigenin synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Cicer arietinum

top print hide
EC NUMBER
COMMENTARY hide
1.14.21.8
-
top print hide
RECOMMENDED NAME
GeneOntology No.
pseudobaptigenin synthase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
calycosin + NADPH + H+ + O2 = pseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
pratensein + NADPH + H+ + O2 = 5-hydroxypseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(-)-maackiain biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
calycosin,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming)
A heme-thiolate enzyme (P-450) catalysing an oxidative reaction that does not incorporate oxygen into the product. Catalyses a step in the biosynthesis of (-)-maackiain, the main pterocarpan phytoalexin in chickpea (Cicer arietinum).
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
calycosin + NADPH + H+ + O2
pseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
pratensein + NADPH + H+ + O2
5-hydroxypseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
NADH leads to a turnover of less than 5% of NADPH
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
BAS 110
-
-
CO
-
complete inhibition
cytochrome c
-
-
juglone
-
-
ketoconazole
-
-
N2/O2
-
N2/O2 atmosphere (9:1) 15-24% inhibition
Plumbagin
-
-
Tetcyclacis
-
-
Triadimefone
-
-
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O2
-
absolutely required for reaction
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
Calycosin
-
pH 7.5, 12°C
0.025 - 0.07
NADPH
0.0012
Pratensein
-
pH 7.5, 12°C
top print hide
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35 - 0.4
BAS 110
0.0025
cytochrome c
Cicer arietinum
-
pH 7.5, 12°C
0.001
juglone
Cicer arietinum
-
pH 7.5, 12°C
0.04 - 0.06
ketoconazole
0.0045 - 0.012
Plumbagin
0.09 - 0.12
Tetcyclacis
0.36 - 0.4
Triadimefone
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12
-
assay at, facilitates detection of the reactions and yields more product
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.21.8)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)