Information on EC 1.14.21.8 - pseudobaptigenin synthase

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The expected taxonomic range for this enzyme is: Cicer arietinum

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EC NUMBER
COMMENTARY hide
1.14.21.8
-
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RECOMMENDED NAME
GeneOntology No.
pseudobaptigenin synthase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
calycosin + NADPH + H+ + O2 = pseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
pratensein + NADPH + H+ + O2 = 5-hydroxypseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
maackiain biosynthesis
-
-
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SYSTEMATIC NAME
IUBMB Comments
calycosin,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming)
A heme-thiolate enzyme (P-450) catalysing an oxidative reaction that does not incorporate oxygen into the product. Catalyses a step in the biosynthesis of (-)-maackiain, the main pterocarpan phytoalexin in chickpea (Cicer arietinum).
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
calycosin + NADPH + H+ + O2
pseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
pratensein + NADPH + H+ + O2
5-hydroxypseudobaptigenin + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
NADH leads to a turnover of less than 5% of NADPH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
BAS 110
-
-
CO
-
complete inhibition
cytochrome c
-
-
juglone
-
-
ketoconazole
-
-
N2/O2
-
N2/O2 atmosphere (9:1) 15-24% inhibition
-
Plumbagin
-
-
Tetcyclacis
-
-
Triadimefone
-
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O2
-
absolutely required for reaction
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
Calycosin
-
pH 7.5, 12°C
0.025 - 0.07
NADPH
0.0012
Pratensein
-
pH 7.5, 12°C
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35 - 0.4
BAS 110
0.0025
cytochrome c
Cicer arietinum
-
pH 7.5, 12°C
0.001
juglone
Cicer arietinum
-
pH 7.5, 12°C
0.04 - 0.06
ketoconazole
0.0045 - 0.012
Plumbagin
0.09 - 0.12
Tetcyclacis
0.36 - 0.4
Triadimefone
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12
-
assay at, facilitates detection of the reactions and yields more product
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.21.8)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)