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Information on EC 1.14.21.8 - pseudobaptigenin synthase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Cicer arietinum
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pseudobaptigenin synthase
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calycosin + NADPH + H+ + O2 = pseudobaptigenin + NADP+ + 2 H2O
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pratensein + NADPH + H+ + O2 = 5-hydroxypseudobaptigenin + NADP+ + 2 H2O
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(-)-maackiain biosynthesis
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calycosin,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming)
A heme-thiolate enzyme (P-450) catalysing an oxidative reaction that does not incorporate oxygen into the product. Catalyses a step in the biosynthesis of (-)-maackiain, the main pterocarpan phytoalexin in chickpea (Cicer arietinum).
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5-hydroxypseudobaptigenin synthase
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pseudobaptigenin synthase
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brenda
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calycosin + NADPH + H+ + O2
pseudobaptigenin + NADP+ + 2 H2O
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pratensein + NADPH + H+ + O2
5-hydroxypseudobaptigenin + NADP+ + 2 H2O
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NADPH
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NADH leads to a turnover of less than 5% of NADPH
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N2/O2
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N2/O2 atmosphere (9:1) 15-24% inhibition
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O2
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absolutely required for reaction
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0.002
Calycosin
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pH 7.5, 12°C
0.0012
Pratensein
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pH 7.5, 12°C
0.025
NADPH
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pH 7.5, 12°C, 5-hydroxypseudobaptigenin synthesis
0.07
NADPH
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pH 7.5, 12°C, pseudobaptigenin synthesis
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0.0025
cytochrome c
Cicer arietinum
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pH 7.5, 12°C
0.001
juglone
Cicer arietinum
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pH 7.5, 12°C
0.35
BAS 110
Cicer arietinum
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pH 7.5, 12°C
0.4
BAS 110
Cicer arietinum
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pH 7.5, 12°C
0.04
ketoconazole
Cicer arietinum
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pH 7.5, 12°C
0.06
ketoconazole
Cicer arietinum
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pH 7.5, 12°C
0.0045
Plumbagin
Cicer arietinum
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pH 7.5, 12°C
0.012
Plumbagin
Cicer arietinum
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pH 7.5, 12°C
0.09
Tetcyclacis
Cicer arietinum
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pH 7.5, 12°C
0.12
Tetcyclacis
Cicer arietinum
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pH 7.5, 12°C
0.36
Triadimefone
Cicer arietinum
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pH 7.5, 12°C
0.4
Triadimefone
Cicer arietinum
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pH 7.5, 12°C
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12
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assay at, facilitates detection of the reactions and yields more product
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brenda
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Clemens, S.; Barz, W.
Cytochrome P450-dependent methylenedioxy bridge formation in Cicer arietinum
Phytochemistry
41
457-460
1996
Cicer arietinum
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brenda
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