Information on EC 1.14.19.21 - cholesterol 7-desaturase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.19.21
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RECOMMENDED NAME
GeneOntology No.
cholesterol 7-desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cholesterol + O2 + NAD(P)H + H+ = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ecdysone and 20-hydroxyecdysone biosynthesis
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Insect hormone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
cholesterol,NAD(P)H:oxygen oxidoreductase (7,8 dehydrogenating)
The enzyme, characterized from several organisms including the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the ciliate Tetrahymena thermophila, is a Rieske oxygenase. In insects it participates in the the biosythesis of ecdysteroid hormones. The electrons are transferred from NAD(P)H via an electron transfer chain likely to include ferredoxin reductase and ferredoxin. The enzyme differs from regular desaturases, such as EC 1.14.19.20, 7-sterol 5(6)-desaturase, which are cytochrome b5-dependent and contain the three His-boxes that are typical to most desaturases.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme regulates DAF-12 activities for reproductive development and longevity by controlling dafachronic acids production
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
22-cholesten-3beta-ol + NADH + H+
7,22-cholestadiene-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
22-cholesten-3beta-ol NADPH + H+
7,22-cholestadiene-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
5alpha-colesta-7,22-dien-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
5alpha-colesta-7,22-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
5alpha-cholestan-3beta-ol + O2 + NADH + H+
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
cholestan-3beta-ol + NADH + H+
7-cholesten-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
cholestan-3beta-ol + NADPH + H+
7-cholesten-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
cholestanol + O2 + NADPH + H+
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
show the reaction diagram
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main product
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?
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NAP+ + H2O
show the reaction diagram
cholesterol + O2 + NADPH + H+
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
stigmastanol + O2 + NADPH + H+
5alpha-24-ethylcholest-7-en-3beta-ol + NADP+ + H2O
show the reaction diagram
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main product
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?
stigmasterol + O2 + NADPH + H+
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
show the reaction diagram
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main product
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
22-cholesten-3beta-ol + NADH + H+
7,22-cholestadiene-3beta-ol + NAD+ + H2O
show the reaction diagram
I7ML19
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-
-
?
22-cholesten-3beta-ol NADPH + H+
7,22-cholestadiene-3beta-ol + NADP+ + H2O
show the reaction diagram
I7ML19
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-
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?
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
5alpha-colesta-7,22-dien-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
5alpha-colesta-7,22-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
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-
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?
5alpha-cholestan-3beta-ol + O2 + NADH + H+
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
show the reaction diagram
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?
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
show the reaction diagram
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?
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
show the reaction diagram
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-
-
-
?
cholestan-3beta-ol + NADH + H+
7-cholesten-3beta-ol + NAD+ + H2O
show the reaction diagram
I7ML19
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-
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?
cholestan-3beta-ol + NADPH + H+
7-cholesten-3beta-ol + NADP+ + H2O
show the reaction diagram
I7ML19
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?
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
show the reaction diagram
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-
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?
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
cholesterol + O2 + NADH + H+
cholesta-5,7-dien-3beta-ol + NAP+ + H2O
show the reaction diagram
cholesterol + O2 + NADPH + H+
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 63000, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Drosophila melanogaster S2 cells
expressed in Sf9 cells
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expressed in Sf9 insect cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H190A
inactive
H282A
inactive
C178A
inactive