Information on EC 1.14.19.11 - acyl-[acyl-carrier-protein] 4-desaturase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.19.11
-
RECOMMENDED NAME
GeneOntology No.
acyl-[acyl-carrier-protein] 4-desaturase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = (4Z)-hexadec-4-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
petroselinate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
palmitoyl-[acyl-carrier protein],reduced acceptor:oxygen oxidoreductase (4,5 cis-dehydrogenating)
The enzymes from the plants Coriandrum sativum (coriander) and Hedera helix (English ivy) are involved in biosynthesis of petroselinate [(6Z)-octadec-6-enoate], which is formed by elongation of (4Z)-hexadec-4-enoate. The ivy enzyme can also act on oleoyl-[acyl-carrier protein] and palmitoleoyl-[acyl-carrier protein], generating the corresponding 4,9-diene.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
English ivy
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
?
show the reaction diagram
-
desaturation occurs at the DELTA9 position
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
?
show the reaction diagram
-
desaturation occurs at the DELTA9 position
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
oleoyl-[acyl-carrier protein]
-
pH and temperature not specified in the publication
0.0021
palmitoleoyl-[acyl-carrier protein]
-
pH and temperature not specified in the publication
0.0015
palmitoyl-[acyl-carrier protein]
-
pH and temperature not specified in the publication
0.0015
stearoyl-[acyl-carrier protein]
-
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
oleoyl-[acyl-carrier protein]
Hedera helix
-
pH and temperature not specified in the publication
0.048
palmitoleoyl-[acyl-carrier protein]
Hedera helix
-
pH and temperature not specified in the publication
0.25
palmitoyl-[acyl-carrier protein]
Hedera helix
-
pH and temperature not specified in the publication
0.17
stearoyl-[acyl-carrier protein]
Hedera helix
-
pH and temperature not specified in the publication
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
x * 36000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 36000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Poros 20 CM column chromatography and G-300SW gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli BL21 cells and leaf discs of Nicotiana tabacum
-
expressed in Escherichia coli BL21 DE3 Gold cells and in Arabidopsis thaliana
-
expressed in Nicotiana tabacum cells
-