Information on EC 1.14.17.B1 - N-methylcoclaurine 3'-monooxygenase (ascorbate)

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The expected taxonomic range for this enzyme is: Berberis stolonifera

EC NUMBER
COMMENTARY hide
1.14.17.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
N-methylcoclaurine 3'-monooxygenase (ascorbate)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-N-methylcoclaurine + ascorbate + O2 = (S)-3'-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-N-methylcoclaurine,ascorbate:oxygen oxidoreductase (3'-hydroxylating)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-coclaurine + ascorbate + O2
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
(S)-coclaurine + ascorbate + O2
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
4-methylcatechol + ascorbate + O2
4-methyl-o-benzoquinone + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
L-tyrosine + ascorbate + O2
L-dopa + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
N-methylcoclaurine + ascorbate + O2
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
tyramine + ascorbate + O2
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
azide
-
12 mM, 50% inhibition
Bathocuproine
-
-
CN-
-
1 mM, complete inhibition
diethyldithiocarbamate
-
0.3 mM, complete inhibition
dipicolinic acid
-
-
EDTA
-
no inhibition EDTA
Thiourea
-
13 mM, 50% inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.065
-
with tyrosine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
-
50% of maximal activity at pH 4.5 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 36000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
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