Information on EC 1.14.15.14 - methyl-branched lipid omega-hydroxylase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
1.14.15.14
-
RECOMMENDED NAME
GeneOntology No.
methyl-branched lipid omega-hydroxylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a methyl-branched lipid + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = an omega-hydroxy-methyl-branched lipid + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
SYSTEMATIC NAME
IUBMB Comments
methyl-branched lipid,reduced-ferredoxin:oxygen oxidoreductase (omega-hydroxylating)
The enzyme, found in pathogenic and nonpathogenic mycobacteria species, actinomycetes, and some proteobacteria, hydroxylates the omega-carbon of a number of methyl-branched lipids, including (2E,6E)-farnesol, phytanate, geranylgeraniol, 15-methylpalmitate and (2E,6E)-farnesyl diphosphate. It is a P-450 heme-thiolate enzyme.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
cholest-4-en-3-one + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
-
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
-
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
-
-
-
?
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
-
-
-
-
?
palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid
-
-
?
phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
15-methylpalmitic acid
at pH 7.4 and 25°C
-
0.036
cholest-4-en-3-one
-
at pH 7.5 and 25°C
0.021 - 0.036
farnesol
0.032
geranylgeraniol
at pH 7.4 and 25°C
0.054
phytanic acid
at pH 7.4 and 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.195
cholest-4-en-3-one
Mycobacterium tuberculosis
-
at pH 7.5 and 25°C
0.258
farnesol
Mycobacterium tuberculosis
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at pH 7.5 and 25°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48700
x * 48700, calculated from amino acid sequence
50000
x * 50000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, free enzyme using 1% (w/v) PEGMME 2000, 0.1 M Na cacodylate (pH 6.8), and 0.9 M succinic acid, and enzyme in complex with phytanic acid using 21% (w/v) PEG 4000, 0.1 M Bis-Tris (pH 5.5), and 0.2 M calcium acetate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells