Information on EC 1.14.13.97 - taurochenodeoxycholate 6alpha-hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.97
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RECOMMENDED NAME
GeneOntology No.
taurochenodeoxycholate 6alpha-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
lithocholate + NADPH + H+ + O2 = hyocholate + NADP+ + H2O
show the reaction diagram
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-
-
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lithocholate + NADPH + H+ + O2 = hyodeoxycholate + NADP+ + H2O
show the reaction diagram
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taurochenodeoxycholate + NADPH + H+ + O2 = taurohyocholate + NADP+ + H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
taurochenodeoxycholate,NADPH:oxygen oxidoreductase (6alpha-hydroxylating)
A heme-thiolate protein (P-450). Requires cytochrome b5 for maximal activity. Acts on taurochenodeoxycholate, taurodeoxycholate and less readily on lithocholate and chenodeoxycholate. In adult pig (Sus scrofa), hyocholic acid replaces cholic acid as a primary bile acid [5].
CAS REGISTRY NUMBER
COMMENTARY hide
105669-85-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
lithocholate + NADPH + H+ + O2
hyocholate + NADP+ + H2O
show the reaction diagram
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activity is low compared to activity with taurochenodeoxycholate
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-
?
taurochenodeoxycholate + NADPH + H+ + O2
taurohyocholate + NADP+ + H2O
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0629
taurochenodeoxycholate
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 53000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6alpha-hydroxylase (CYP4A21) gene: evolution by gene duplication and gene conversion
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transfection into COS cells
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