Information on EC 1.14.13.91 - deoxysarpagine hydroxylase

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The expected taxonomic range for this enzyme is: Rauvolfia serpentina

EC NUMBER
COMMENTARY hide
1.14.13.91
-
RECOMMENDED NAME
GeneOntology No.
deoxysarpagine hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10-deoxysarpagine + NADPH + H+ + O2 = sarpagine + NADP+ + H2O
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Indole alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
10-deoxysarpagine,NADPH:oxygen oxidoreductase (10-hydroxylating)
A heme-thiolate protein (P-450).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-deoxysarpagine + NADPH + O2
sarpagine + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-deoxysarpagine + NADPH + O2
sarpagine + NADP+ + H2O
show the reaction diagram
-
most probably the natural substrate
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
can not be replaced by NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidole
-
1.0 mM, 49% inhibition
BAS 110 W
-
1 mM, complete inhibition
BAS 111 W
-
1 mM, 84% inhibition
CO
-
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
cytochrome c
-
0.01 mM, 46% inhibition
ketoconazole
-
1 mM, 69% inhibition
LAB 1500978
-
1 mM, 74% inhibition
Metyrapone
-
1 mM, 83% inhibition
NADP+
-
competitive inhibition
Tetcyclacis
-
1 mM, 45% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0074
10-deoxysarpagine
-
35°C, PH 8.0
0.025
NADPH
-
35°C, PH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-25°C, 20% sucrose, more than 3 months, no loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE