Information on EC 1.14.13.79 - ent-kaurenoic acid oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.79
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RECOMMENDED NAME
GeneOntology No.
ent-kaurenoic acid oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2 = gibberellin A12 aldehyde + NADP+ + 2 H2O
show the reaction diagram
second step, The second step includes a ring-B contraction giving the gibbane skeleton
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ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2 = gibberellin A12 + 3 NADP+ + 4 H2O
show the reaction diagram
overall reaction
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ent-kaur-16-en-19-oate + NADPH + H+ + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
show the reaction diagram
first step
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gibberellin A12 aldehyde + NADPH + H+ + O2 = gibberellin A12 + NADP+ + H2O
show the reaction diagram
third step
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Diterpenoid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating)
Requires cytochrome P-450. Catalyses three sucessive oxidations of ent-kaurenoic acid. The second step includes a ring-B contraction giving the gibbane skeleton. In pumpkin (Cucurbita maxima) ent-6alpha,7alpha-dihydroxykaur-16-en-19-oate is also formed.
CAS REGISTRY NUMBER
COMMENTARY hide
337507-95-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Kao fragment (Kao3), Aegilops tauschii; Tausch's goatgrass, diploid
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
dwarf mutant of barley that accumulates ent-keurenoic acid in developing grains
Uniprot
Manually annotated by BRENDA team
cv. Grand Rapids, gene LsKAO encoding a ent-kaurenoic acid oxidase
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Kao fragment (Kao3), Triticum urartu; diploid
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2
gibberellin A12 aldehyde + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
gibberellin A12 aldehyde + NADP+ + 2 H2O
show the reaction diagram
ent-kaur-16-en-19-oate + NADPH + H+ + O2
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
show the reaction diagram
ent-kaur-16-en-19-oate + NADPH + O2
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
show the reaction diagram
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?
gibberellin A12 aldehyde + NADPH + O2
gibberellin A12 + NADP+ + H2O
show the reaction diagram
additional information
?
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the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins, gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2
gibberellin A12 aldehyde + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
gibberellin A12 aldehyde + NADP+ + 2 H2O
show the reaction diagram
ent-kaur-16-en-19-oate + NADPH + H+ + O2
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
show the reaction diagram
ent-kaur-16-en-19-oate + NADPH + O2
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
show the reaction diagram
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?
gibberellin A12 aldehyde + NADPH + O2
gibberellin A12 + NADP+ + H2O
show the reaction diagram
additional information
?
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B5MEX7
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins, gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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NADPH
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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expression of the gene PsKAO1
Manually annotated by BRENDA team
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expression of the gene PsKAO1
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
both genes PsKAO1 and PsKAO2 encode the ent-kaurenoic acid oxidase, expression in Saccharomyces cerevisiae
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expression in yeast
gene LsKAO, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
PCR-amplification
PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification; PCR-amplification