Information on EC 1.14.13.75 - vinorine hydroxylase

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The expected taxonomic range for this enzyme is: Rauvolfia serpentina

EC NUMBER
COMMENTARY hide
1.14.13.75
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RECOMMENDED NAME
GeneOntology No.
vinorine hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
vinorine + NADPH + H+ + O2 = vomilenine + NADP+ + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
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Biosynthesis of secondary metabolites
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Indole alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
vinorine,NADPH:oxygen oxidoreductase (21alpha-hydroxylating)
A heme-thiolate protein (P-450). Forms a stage in the biosynthesis of the indole alkaloid ajmaline.
CAS REGISTRY NUMBER
COMMENTARY hide
162875-03-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
vinorine + NADPH + H+ + O2
vomilenine + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
vinorine + NADPH + H+ + O2
vomilenine + NADP+ + H2O
show the reaction diagram
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enzyme is involved in the biosynthesis of the alkaloid ajmaline
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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strictly dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
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ketoconazole
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26
vinorine
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-28°C, 20% sucrose, no loss of activity
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