Information on EC 1.14.13.62 - 4-hydroxyquinoline 3-monooxygenase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.14.13.62
-
RECOMMENDED NAME
GeneOntology No.
4-hydroxyquinoline 3-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
quinolin-4-ol + NADH + H+ + O2 = quinolin-3,4-diol + NAD+ + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
quinolin-4(1H)-one,NADH:oxygen oxidoreductase (3-oxygenating)
Quinolin-4-ol exists largely as the quinolin-4(1H)-one tautomer.
CAS REGISTRY NUMBER
COMMENTARY hide
144378-37-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 33/1
-
-
Manually annotated by BRENDA team
strain 33/1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1H-4-Oxoquinoline + NADH + O2
1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O
show the reaction diagram
1H-4-Oxoquinoline + NADPH + O2
1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
required as cofactor
NADPH
-
50% of the activity compared to NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
-
partial inhibition
iodoacetate
-
partial inhibition
Sodium dithionite
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
-
15%, 1.2fold activation. Strong decrease in activity above 15%
ethanol
-
15%, 1.7fold activation. Strong decrease in activity above 15%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
1H-4-Oxoquinoline
-
-
0.087
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
3 * 42000, SDS-PAGE
126000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
strong inactivation above
7423
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% loss of enzyme activity after 96 h without additions
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
Ethanol
Ethylene glycol
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE