Information on EC 1.14.13.43 - questin monooxygenase

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The expected taxonomic range for this enzyme is: Aspergillus terreus

EC NUMBER
COMMENTARY hide
1.14.13.43
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RECOMMENDED NAME
GeneOntology No.
questin monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
questin + NADPH + H+ + O2 = demethylsulochrin + NADP+
show the reaction diagram
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
geodin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
questin,NADPH:oxygen oxidoreductase (hydroxylating, anthraquinone-ring-opening)
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin.
CAS REGISTRY NUMBER
COMMENTARY hide
115232-45-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
questin + NADPH + H+ + O2
demethylsulochrin + NADP+
show the reaction diagram
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absolute requirement for oxygen and NADPH
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
questin + NADPH + H+ + O2
demethylsulochrin + NADP+
show the reaction diagram
-
absolute requirement for oxygen and NADPH
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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highly specific
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03
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crude extract
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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complete loss of activity overnight
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very unstable, polyols, non-ionic detergents and EDTA have stabilizing effects
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
cold room, 0.01 M Tris-HCl, pH 7.5, 50% ethylene glycol, v/v, 1 mM EDTA, 0.05% Tween 80, w/v, 70-80% of its original activity after 2 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, additional functional protein or proteins required for full activity
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