Information on EC 1.14.13.4 - melilotate 3-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.4
-
RECOMMENDED NAME
GeneOntology No.
melilotate 3-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-(2-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phenylalanine metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (3-hydroxylating)
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
37256-72-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(2-hydroxyphenyl)propanoate + NADH + O2
3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
show the reaction diagram
additional information
?
-
-
high specificity, low activity with 3-hydroxyphenylpropanoate with 20% of melilotate reduction, phenylpropanoate with 1-2% of meliotate reduction
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-(2-hydroxyphenyl)propanoate + NADH + O2
3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
show the reaction diagram
-
i.e. melilotate, role in metabolism of coumarin
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
-
N-ethylmaleimide
-
-
p-chloromercuribenzoate
-
-
additional information
-
not inhibitory: iodoacetic acid, iodoacetamide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027
3-(2-hydroxyphenyl)propanoate
-
-
0.0013 - 0.086
melilotate
0.0047 - 0.091
NADH
0.02 - 0.05
O2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.87 - 4.48
3-(2-hydroxyphenyl)propanoate
Pseudomonas sp.
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
at pH 5.0 and 9.0: about 30% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
238000 - 250000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is rapidly reduced by irradiation with visible light in presence of EDTA or by dithionite
-
396421
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 1 week
-
-20°C, stable for 3 weeks, highly purified enzyme, concentrated by dialysis
-
-70°C, stable for 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
activity can be restored with FAD
-