Information on EC 1.14.13.220 - resorcinol 4-hydroxylase (NADH)

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The expected taxonomic range for this enzyme is: Rhodococcus jostii

EC NUMBER
COMMENTARY hide
1.14.13.220
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RECOMMENDED NAME
GeneOntology No.
resorcinol 4-hydroxylase (NADH)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
resorcinol + NADH + H+ + O2 = hydroxyquinol + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gamma-resorcylate degradation II
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Benzoate degradation
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SYSTEMATIC NAME
IUBMB Comments
resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
The enzyme, characterized from the bacterium Rhodococcus jostii RHA1, is a single-component hydroxylase. The enzyme has no activity with NADPH. cf. EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH), and EC 1.14.14.27, resorcinol 4-hydroxylase (FADH2).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme gamma-resorcylate catabolic-pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
show the reaction diagram
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the enzyme gamma-resorcylate catabolic-pathway
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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the enzyme has no activity with NADPH
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 59000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcription of tsdB is activated when cells are grown on gamma-resorcylate
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