Information on EC 1.14.13.217 - protodeoxyviolaceinate monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.217
-
RECOMMENDED NAME
GeneOntology No.
protodeoxyviolaceinate monooxygenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protodeoxyviolaceinate + NAD(P)H + H+ + O2 = protoviolaceinate + NAD(P)+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Staurosporine biosynthesis
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
protodeoxyviolaceinate,NAD(P)H:O2 oxidoreductase
The enzyme, characterized from the bacterium Chromobacterium violaceum, participates in the biosynthesis of the violet pigment violacein. The product, protoviolaceinate, is very labile in the presence of oxygen, resulting in the generation of the shunt product proviolacein.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Duganella sp.
gene vioD
-
-
Manually annotated by BRENDA team
gene vioD
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
-
genes vioA, vioC and vioD encode nucleotide-dependent monooxygenases
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
protodeoxyviolaceinate + NAD(P)H + H+ + O2
protoviolaceinate + NAD(P)+
show the reaction diagram
protodeoxyviolaceinate + NADPH + H+ + O2
protoviolaceinate + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protodeoxyviolaceinate + NAD(P)H + H+ + O2
protoviolaceinate + NAD(P)+
show the reaction diagram
protodeoxyviolaceinate + NADPH + H+ + O2
protoviolaceinate + NADP+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 7.5-15 mg/ml protein in 20 mM Tris–HCl pH 8.0, 300 mM NaCl, 10% glycerol, with 0.001 ml of reservoir solution containing 3.5 M sodium formate pH 7.0, and equilibration against 0.05 ml of reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) to homogeneity by nickel affinity chromatography and gel filtration in tandem
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
biosynthetic gene cluster vioABCD, DNA sequencing, transposon mutagenesis, and chemical analysis of the pathway intermediates produced heterologously in Escherichia coli
-
gene vioD, cloned under control of the PTAC promoter and inserted into the fuc locus of Escherichia coli strain BW25113, functional.expression of the vioABCE cluster from Chromobacterium violaceum strain ATCC 12472 under control of the inducible araC system and the vioD gene of Janthinobacterium lividum strain DSM 1522, which enables exclusive production of violacein in Escherichia coli K-12 MG1655 dVio-1
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview
gene vioD, recombinant expression of His-tagged enzyme in Escherichia coli
gene vioD, reconstruction of the violacein biosynthesis pathway in Escherichia coli strain BL21 (DE3), recombinant expression of the C-terminally His6-tagged enzyme
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information