Information on EC 1.14.13.197 - dihydromonacolin L hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.197
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RECOMMENDED NAME
GeneOntology No.
dihydromonacolin L hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3alpha-hydroxy-3,5-dihydromonacolin L acid = monacolin L acid + H2O
show the reaction diagram
dihydromonacolin L acid + O2 + NADPH + H+ = 3alpha-hydroxy-3,5-dihydromonacolin L acid + NADP+ + H2O
show the reaction diagram
dihydromonacolin L acid + O2 + NADPH + H+ = monacolin L acid + NADP+ + 2 H2O
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
dihydromonacolin L acid,NADPH:oxygen oxidoreductase (3-hydroxylating)
A heme-thiolate protein (cytochrome P-450). The dehydration of 3alpha-hydroxy-3,5-dihydromonacolin L acid is believed to be spontaneous [1,2]. The enzyme from fungi also catalyses the reaction of EC 1.14.13.198, monacolin L hydroxylase [3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3alpha-hydroxy-3,5-dihydromonacolin L acid
monacolin L acid + H2O
show the reaction diagram
dihydromonacolin L acid + O2 + NADPH + H+
3alpha-hydroxy-3,5-dihydromonacolin L acid + NADP+ + H2O
show the reaction diagram
dihydromonacolin L acid + O2 + NADPH + H+
monacolin L acid + NADP+ + 2 H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dihydromonacolin L acid + O2 + NADPH + H+
3alpha-hydroxy-3,5-dihydromonacolin L acid + NADP+ + H2O
show the reaction diagram
Q9Y7C8
dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.13.198, monacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the lovA gene in C-terminal FLAG-tagged form is cloned in the same vector coding cpr (cytochrome P450 oxidoreductase), and both lovA and cpr are co-expressed in Saccharomyces cerevisiae