Information on EC 1.14.13.193 - oryzalexin D synthase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.13.193
-
RECOMMENDED NAME
GeneOntology No.
oryzalexin D synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-sandaracopimaradien-3beta-ol + NADPH + H+ + O2 = oryzalexin D + NADP+ + H2O
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
oryzalexin D and E biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ent-sandaracopimaradien-3beta-ol,NADPH:oxygen oxidoreductase (oryzalexin D forming)
A heme-thiolate protein (cytochrome P-450). Isolated from Oryza sativa (rice). Oryzalexin D is a phytoalexin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-cassadiene + NADPH + H+ + O2
?
show the reaction diagram
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-
-
-
?
ent-isokaurene + NADPH + H+ + O2
?
show the reaction diagram
-
-
-
-
?
ent-kaurene + NADPH + H+ + O2
?
show the reaction diagram
-
-
-
-
?
ent-pimaradiene + NADPH + H+ + O2
?
show the reaction diagram
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-
-
-
?
ent-sandaracopimaradien-3beta-ol + NADPH + H+ + O2
oryzalexin D + NADP+ + H2O
show the reaction diagram
syn-pimaradiene + NADPH + H+ + O2
momilactone A + NADP+ + H2O
show the reaction diagram
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-
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-
?
additional information
?
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no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-sandaracopimaradien-3beta-ol + NADPH + H+ + O2
oryzalexin D + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
ent-cassadiene
-
pH and temperature not specified in the publication
-
0.015
ent-isokaurene
-
pH and temperature not specified in the publication
0.005
ent-kaurene
-
pH and temperature not specified in the publication
0.004
ent-pimaradiene
-
pH and temperature not specified in the publication
-
0.007
ent-sandaracopimaradien-3beta-ol
-
pH and temperature not specified in the publication
0.002
syn-pimaradiene
-
pH and temperature not specified in the publication
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
ent-cassadiene
Oryza sativa
-
pH and temperature not specified in the publication
-
0.015
ent-isokaurene
Oryza sativa
-
pH and temperature not specified in the publication
0.009
ent-kaurene
0.011
ent-sandaracopimaradien-3beta-ol
Oryza sativa
-
pH and temperature not specified in the publication
0.006
syn-pimaradiene
Oryza sativa
-
pH and temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
ent-cassadiene
Oryza sativa
-
pH and temperature not specified in the publication
197622
1
ent-isokaurene
Oryza sativa
-
pH and temperature not specified in the publication
15211
1.8
ent-kaurene
Oryza sativa
-
pH and temperature not specified in the publication
13281
2.3
ent-pimaradiene
Oryza sativa
-
pH and temperature not specified in the publication
197623
1.6
ent-sandaracopimaradien-3beta-ol
Oryza sativa
-
pH and temperature not specified in the publication
190611
2.6
syn-pimaradiene
Oryza sativa
-
pH and temperature not specified in the publication
197624
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
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