Information on EC 1.14.13.191 - ent-sandaracopimaradiene 3-hydroxylase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.13.191
-
RECOMMENDED NAME
GeneOntology No.
ent-sandaracopimaradiene 3-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-sandaracopimaradiene + NADPH + H+ + O2 = ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
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-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
oryzalexin D and E biosynthesis
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-
diterpene phytoalexins precursors biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ent-sandaracopimaradiene,NADPH:oxygen oxidoreductase (ent-sandaracopimaradien-3beta-ol forming)
A heme-thiolate protein (cytochrome P-450). Isolated from Oryza sativa (rice). Participates in the pathway for the biosynthesis of oryzalexins, a group of related phytoalexins produced by rice.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7beta-hydroxy-ent-sandaracopimaradiene + NADPH + H+ + O2
oryzalexin D + NADP+ + H2O
show the reaction diagram
-
poor reaction
-
-
?
ent-cassadiene + NADPH + H+ + O2
3alpha-hydroxy-ent-cassadiene + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
ent-kaurene + NADPH + H+ + O2
3alpha-hydroxy-ent-kaurene + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-cassadiene + NADPH + H+ + O2
3alpha-hydroxy-ent-cassadiene + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
ent-kaurene + NADPH + H+ + O2
3alpha-hydroxy-ent-kaurene + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
ent-sandaracopimaradiene + NADPH + H+ + O2
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
ent-cassadiene
-
pH and temperature not specified in the publication
0.007
ent-kaurene
-
pH and temperature not specified in the publication
0.0023
ent-sandaracopimaradiene
-
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
ent-cassadiene
Oryza sativa
-
pH and temperature not specified in the publication
0.022
ent-kaurene
Oryza sativa
-
pH and temperature not specified in the publication
0.013
ent-sandaracopimaradiene
Oryza sativa
-
pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9
ent-cassadiene
Oryza sativa
-
pH and temperature not specified in the publication
197622
3
ent-kaurene
Oryza sativa
-
pH and temperature not specified in the publication
13281
6
ent-sandaracopimaradiene
Oryza sativa
-
pH and temperature not specified in the publication
193489
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
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expressed in Escherichia coli strain C41
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