Information on EC 1.14.13.184 - protopanaxadiol 6-hydroxylase

Word Map on EC 1.14.13.184
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The expected taxonomic range for this enzyme is: Panax

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EC NUMBER
COMMENTARY hide
1.14.13.184
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RECOMMENDED NAME
GeneOntology No.
protopanaxadiol 6-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protopanaxadiol + NADPH + H+ + O2 = protopanaxatriol + NADP+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ginsenoside metabolism
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ginsenosides biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
protopanaxadiol,NADPH:O2 oxidoreductase (6alpha-hydroxylating)
A heme-thiolate protein (P-450). Involved in the biosynthetic pathway of ginsenosides. Isolated from the rhizomes of ginseng (Panax ginseng).
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
show the reaction diagram
additional information
?
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very low activity with NADH
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
show the reaction diagram
additional information
?
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very low activity with NADH
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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NADPH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidol
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53.1% residual activity at 0.1 mM
carbon monoxide
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an atmosphere of 100% CO completely inhibits enzyme activity in dark. Inhibition by carbon monoxide is partially reversible upon illumination with blue light
cytochrome c
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32.9% residual activity at 0.1 mM
miconazole
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25.7% residual activity at 0.1 mM
additional information
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activity is not affected by dimethylsulfoxide at 0.1% (v/v)
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phenobarbital
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maximal activity with addition of 0.5 mM phenobarbital
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034
protopanaxadiol
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in 100 mM phosphate buffer (pH 7.6), at 30�C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55300
x * 55300, calculated from amino acid sequence
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55300, calculated from amino acid sequence
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain GY-1
expressed in Saccharomyces cerevisiae WAT21 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is not affected by methyl jasmonate treatment
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.184)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)