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Information on EC 1.14.13.184 - protopanaxadiol 6-hydroxylase Word Map on EC 1.14.13.184
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The expected taxonomic range for this enzyme is: Panax
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protopanaxadiol 6-hydroxylase
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protopanaxadiol + NADPH + H+ + O2 = protopanaxatriol + NADP+ + H2O
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ginsenoside metabolism
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ginsenosides biosynthesis
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protopanaxadiol,NADPH:O2 oxidoreductase (6alpha-hydroxylating)
A heme-thiolate protein (P-450). Involved in the biosynthetic pathway of ginsenosides. Isolated from the rhizomes of ginseng (Panax ginseng).
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protopanaxatriol synthase
CYP716A53v2
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P6H
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protopanaxatriol synthase
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protopanaxatriol synthase
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UniProt
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UniProt
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metabolism
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protopanaxadiol 6-hydroxylase is a key enzyme in regulating Rg-group ginsenoside biosynthesis in Panax notoginseng cells
metabolism
protopanaxadiol 6-hydroxylase produces protopanaxatriol from protopanaxadiol, which is an important step in the formation of dammarane-type triterpene aglycones in ginseng saponin biosynthesis
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
additional information
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very low activity with NADH
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
additional information
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very low activity with NADH
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
I7CT85
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
I7C6E8
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protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
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NADPH
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Ancymidol
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53.1% residual activity at 0.1 mM
carbon monoxide
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an atmosphere of 100% CO completely inhibits enzyme activity in dark. Inhibition by carbon monoxide is partially reversible upon illumination with blue light
cytochrome c
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32.9% residual activity at 0.1 mM
miconazole
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25.7% residual activity at 0.1 mM
additional information
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activity is not affected by dimethylsulfoxide at 0.1% (v/v)
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Phenobarbital
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maximal activity with addition of 0.5 mM phenobarbital
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0.034
protopanaxadiol
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in 100 mM phosphate buffer (pH 7.6), at 30°C
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highest expression
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55300
x * 55300, calculated from amino acid sequence
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x * 55300, calculated from amino acid sequence
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expressed in Saccharomyces cerevisiae strain GY-1
expressed in Saccharomyces cerevisiae WAT21 cells
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the enzyme is not affected by methyl jasmonate treatment
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C7A53_PANGI
469
53297
Swiss-Prot
C7A52_PANGI
481
53955
Swiss-Prot
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Yue, C.J.; Zhou, X.; Zhong, J.J.
Protopanaxadiol 6-hydroxylase and its role in regulating the ginsenoside heterogeneity in Panax notoginseng cells
Biotechnol. Bioeng.
100
933-940
2008
Panax notoginseng
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Han, J.Y.; Hwang, H.S.; Choi, S.W.; Kim, H.J.; Choi, Y.E.
Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng
Plant Cell Physiol.
53
1535-1545
2012
Panax ginseng (I7C6E8), Panax ginseng
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Dai, Z.; Wang, B.; Liu, Y.; Shi, M.; Wang, D.; Zhang, X.; Liu, T.; Huang, L.; Zhang, X.
Producing aglycons of ginsenosides in bakers yeast
Sci. Rep.
4
3698
2014
Panax ginseng (I7CT85), Panax ginseng
brenda
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