Information on EC 1.14.13.18 - 4-hydroxyphenylacetate 1-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.18
-
RECOMMENDED NAME
GeneOntology No.
4-hydroxyphenylacetate 1-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 = homogentisate + NAD(P)+ + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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-
Tyrosine metabolism
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SYSTEMATIC NAME
IUBMB Comments
4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating)
A flavoprotein (FAD). Also acts on 4-hydroxyhydratropate (forming 2-methylhomogentisate) and on 4-hydroxyphenoxyacetate (forming hydroquinone and glycolate).
CAS REGISTRY NUMBER
COMMENTARY hide
55326-44-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PHPXAa-B
-
-
Manually annotated by BRENDA team
Bacillus sp. PHPXAa-B
PHPXAa-B
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
CBS3
-
-
Manually annotated by BRENDA team
CBS3
-
-
Manually annotated by BRENDA team
124X
-
-
Manually annotated by BRENDA team
124X
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxy-2-methylphenylacetate + NAD(P)H + O2
?
show the reaction diagram
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-
-
-
?
4-hydroxyhydratropate + NAD(P)H + O2
2-methylhomogentisate + NAD(P)+ + H2O
show the reaction diagram
-
-
-
?
4-hydroxyphenoxyacetate + ?
hydroquinone + glycolate + ?
show the reaction diagram
-
-
-
?
4-hydroxyphenylacetate + NAD(P)H + O2
homogentisate + NAD(P)+ + H2O
show the reaction diagram
additional information
?
-
-
low activity with: 4-hydroxyphenylpropionate, 4-hydroxy-3-methylphenylacetate, 3,4-dihydroxyphenylacetate
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,4-dihydroxyphenylacetic acid
-
-
4-Hydroxy-3-methylphenylacetic acid
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-
4-hydroxyphenylpropionic acid
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DL-4-hydroxymandelic acid
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031
4-hydroxyphenylacetate
-
-
0.095
NADH
-
-
0.25
NADPH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in the presence of dithioerythritol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE