Information on EC 1.14.13.172 - salicylate 5-hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.172
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RECOMMENDED NAME
GeneOntology No.
salicylate 5-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Naphthalene degradation
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salicylate degradation II
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salicylate glucosides biosynthesis II
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SYSTEMATIC NAME
IUBMB Comments
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
large oxygenase component and small oxagenase component
O52379 and O52380
UniProt
Manually annotated by BRENDA team
large oxygenase component and small oxagenase component
O52379 and O52380
UniProt
Manually annotated by BRENDA team
large oxygenase component and small oxagenase component
O52379 and O52380
UniProt
Manually annotated by BRENDA team
large oxygenase component and small oxagenase component
O52379 and O52380
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dihydroxybenzoate + NADH + H+ + O2
?
show the reaction diagram
2,6-dihydroxybenzoate + NADH + H+ + O2
?
show the reaction diagram
2-nitrophenol + NADH + H+ + O2
?
show the reaction diagram
salicylate + NADH + H+ + O2
2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
salicylate + NADPH + H+ + O2
2,5-dihydroxybenzoate + NADP+ + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
salicylate + NADH + H+ + O2
2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
salicylate + NADPH + H+ + O2
2,5-dihydroxybenzoate + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
[2Fe-2S]-center
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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the enzyme contains one Rieske-type [2Fe-2S] cluster and 7.5 g iron per mol enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9398
2,6-dihydroxybenzoate
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in 50 mM MES buffer (pH 6.4), at 22C
0.3284
2-Nitrophenol
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in 50 mM MES buffer (pH 6.4), at 22C
0.05976
NADH
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in 50 mM MES buffer (pH 6.4), at 22C
0.05641
NADPH
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in 50 mM MES buffer (pH 6.4), at 22C
0.1071
salicylate
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in 50 mM MES buffer (pH 6.4), at 22C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.051
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in 50 mM MES buffer, pH 6.4, at 30C
107.1
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after 124fold purification, in 50 mM MES buffer (pH 6.4), at 22C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
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3 * 52000 + 3 * 20000, His6-tagged enzyme, SDS-PAGE
45000
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4 * 45000, SDS-PAGE
48799
O52379 and O52380
x * 48799 + x * 18800, calculated from amino acid sequence
51000
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3 * 51000 + 3 * 18800, His6-tagged enzyme, calculated from amino acid sequence
52000
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3 * 52000 + 3 * 20000, His6-tagged enzyme, SDS-PAGE
180000
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gel filtration
195200
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterohexamer
homotetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the three-component Fe-S protein complex (NagAaGHAb) of the enzyme is purified by HisTrap column chromatography and Sephadex G-25 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3)/pLysS cells
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expressed in Escherichia coli DH5alpha cells
O52379 and O52380