Information on EC 1.14.13.168 - indole-3-pyruvate monooxygenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.14.13.168
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RECOMMENDED NAME
GeneOntology No.
indole-3-pyruvate monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(indol-3-yl)pyruvate + NADPH + H+ + O2 = (indol-3-yl)acetate + NADP+ + H2O + CO2
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetate biosynthesis I
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indole-3-acetate biosynthesis II
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Tryptophan metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
indole-3-pyruvate,NADPH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
This plant enzyme, along with EC 2.6.1.99 L-tryptophan---pyruvate aminotransferase, is responsible for the biosynthesis of the plant hormone indole-3-acetate from L-tryptophan.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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a single YUC gene in Arabidopsis does not cause obvious developmental defects, but simultaneous disruption of several YUC genes in Arabidopsis leads to defects in embryogenesis, seedling growth, flower development, and vascular pattern
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)pyruvate + NADPH + H+ + O2
(indol-3-yl)acetate + NADP+ + H2O + CO2
show the reaction diagram
phenylpyruvate + NADPH + H+ + O2
phenylacetate + NADP+ + H2O + CO2
show the reaction diagram
isoform YUC6 shows basal NADPH oxidase activity in the absence of the cosubstrate phenylpyruvate
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
; isoform YUC6 contains oxidized flavin as a cofactor
additional information
isoform YUC6 does not accept FMN as cofactor
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
NADPH
in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C
0.043
phenylpyruvate
in the presence of NADPH, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31
NADPH
Arabidopsis thaliana
Q8VZ59
in the presence of phenylpyruvate, in 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, and 30% (v/v) glycerol, at 25°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 48000, isoform YUC6, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expession in Escherichia coli; expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli
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