Information on EC 1.14.13.150 - alpha-humulene 10-hydroxylase

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The expected taxonomic range for this enzyme is: Zingiber zerumbet

EC NUMBER
COMMENTARY hide
1.14.13.150
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RECOMMENDED NAME
GeneOntology No.
alpha-humulene 10-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-humulene + O2 + NADPH + H+ = 10-hydroxy-alpha-humulene + NADP+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
zerumbone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
alpha-humulene,NADPH:oxygen 10-oxidoreductase
Requires cytochrome P-450. The recommended numbering of humulene gives 10-hydroxy-alpha-humulene as the product rather than 8-hydroxy-alpha-humulene as used by the reference. See {iupac/sectionF/index::Section F: Natural Product Nomenclature}.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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the enzyme belongs to the cytochrome P450 monooxygenases
metabolism
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CYP71BA1 catalyzes the second step of zerumbone biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-humulene + O2 + NADPH + H+
10-hydroxy-alpha-humulene + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-humulene + O2 + NADPH + H+
10-hydroxy-alpha-humulene + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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cytochrome P450 enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression
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functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information