Information on EC 1.14.13.146 - taxoid 14beta-hydroxylase

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The expected taxonomic range for this enzyme is: Taxus

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EC NUMBER
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1.14.13.146
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RECOMMENDED NAME
GeneOntology No.
taxoid 14beta-hydroxylase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + O2 + NADPH + H+ = 10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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taxol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
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ORGANISM
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LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
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LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+
5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O
show the reaction diagram
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?
5alpha-acetoxytaxadiene + O2 + NADPH + H+
? + NADP+ + H2O
show the reaction diagram
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?
5alpha-hydroxytaxa-4(20),11(12)-diene + O2 + NADPH + H+
? + NADP+ + H2O
show the reaction diagram
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?
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COFACTOR
ORGANISM
UNIPROT
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LITERATURE
IMAGE
cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
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LITERATURE
IMAGE
0.055
5alpha-acetoxy-10beta-hydroxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
0.033
5alpha-acetoxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
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LOCALIZATION
ORGANISM
UNIPROT
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GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
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LITERATURE
57146
x * 57146, calculated from amino acid sequence
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SUBUNITS
ORGANISM
UNIPROT
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LITERATURE
?
x * 57146, calculated from amino acid sequence
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT11 cells
expressed in the Taxus x media TM3 cell line. The expression of endogenous taxoid 14beta-hydroxylase gene is significantly suppressed by the exogenous as taxoid 14beta-hydroxylase gene
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APPLICATION
ORGANISM
UNIPROT
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LITERATURE
biotechnology
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an antisense suppression approach, repressing the expression of the taxoid 14beta-hydroxlyase gene in yew cell cultures, is useful to inhibit the expression of other important genes in side-route of Taxol pathway and this may diverts the flow of taxadiene mainly towards Taxol
medicine
manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.146)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)