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Information on EC 1.14.13.146 - taxoid 14beta-hydroxylase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Taxus
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taxoid 14beta-hydroxylase
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10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + O2 + NADPH + H+ = 10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O
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Diterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
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cytochrome P450 taxoid 14beta-hydroxylase
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taxoid 14beta-hydroxylase
taxoid 14beta-hydroxylase
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taxoid 14beta-hydroxylase
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UniProt
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brenda
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5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+
5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O
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5alpha-acetoxytaxadiene + O2 + NADPH + H+
? + NADP+ + H2O
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5alpha-hydroxytaxa-4(20),11(12)-diene + O2 + NADPH + H+
? + NADP+ + H2O
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cytochrome P450
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cytochrome P450
dependent on
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0.055
5alpha-acetoxy-10beta-hydroxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
0.033
5alpha-acetoxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
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57146
x * 57146, calculated from amino acid sequence
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x * 57146, calculated from amino acid sequence
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expressed in Saccharomyces cerevisiae WAT11 cells
expressed in the Taxus x media TM3 cell line. The expression of endogenous taxoid 14beta-hydroxylase gene is significantly suppressed by the exogenous as taxoid 14beta-hydroxylase gene
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biotechnology
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an antisense suppression approach, repressing the expression of the taxoid 14beta-hydroxlyase gene in yew cell cultures, is useful to inhibit the expression of other important genes in side-route of Taxol pathway and this may diverts the flow of taxadiene mainly towards Taxol
medicine
manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents
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T14H_TAXCU
509
57147
Swiss-Prot
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Li, F.; Ma, X.; Hu, X.; Hoffman, A.; Dai, J.; Qiu, D.
Antisense-induced suppression of taxoid 14beta-hydroxylase gene expression in transgenic Taxus x media cells
Afr. J. Biotechnol.
10
8720-8728
2011
Taxus wallichiana var. chinensis
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Jennewein, S.; Rithner, C.D.; Williams, R.M.; Croteau, R.
Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase
Arch. Biochem. Biophys.
413
262-270
2003
Taxus cuspidata (Q84KI1)
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