Information on EC 1.14.13.145 - ent-cassa-12,15-diene 11-hydroxylase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.13.145
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RECOMMENDED NAME
GeneOntology No.
ent-cassa-12,15-diene 11-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-cassa-12,15-diene + O2 + NADPH + H+ = ent-11beta-hydroxycassa-12,15-diene + NADP+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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phytocassanes biosynthesis, shared reactions
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SYSTEMATIC NAME
IUBMB Comments
ent-cassa-12,15-diene,NADPH:oxygen 11-oxidoreductase
Requires cytochrome P450. A rice, Oryza sativa, enzyme involved in the biosynthesis of the antifungal phytocassanes.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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RNAi-mediated double knockdown of CYP76M7 and -8 suppresses elicitor inducible phytocassane production, indicating a role for these monooxygenases in phytocassane biosynthesis
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-cassa-12,15-diene + O2 + NADPH + H+
11-(S)-hydroxy-entcassa-12,15-diene + H2O + NADP+
show the reaction diagram
specific substrate, C11alpha hydroxylation
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?
additional information
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only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.039
ent-cassa-12,15-diene
in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.13
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain C41 and in Sf21 insect cells