Information on EC 1.14.13.144 - 9beta-pimara-7,15-diene oxidase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.13.144
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RECOMMENDED NAME
GeneOntology No.
9beta-pimara-7,15-diene oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9beta-pimara-7,15-dien-19-al + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-oate + NADP+ + H2O
show the reaction diagram
(1c)
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9beta-pimara-7,15-dien-19-ol + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-al + NADP+ + 2 H2O
show the reaction diagram
(1b)
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9beta-pimara-7,15-diene + 3 O2 + 3 NADPH + 3 H+ = 9beta-pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O
show the reaction diagram
overall reaction
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9beta-pimara-7,15-diene + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-ol + NADP+ + H2O
show the reaction diagram
(1a)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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diterpene phytoalexins precursors biosynthesis
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Diterpenoid biosynthesis
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momilactone A biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase
Requires cytochrome P-450. A rice, Oryza sativa, enzyme involved in the phytoalexin momilactone biosynthesis. It also acts similarly on 9beta-stemod-13(17)-ene.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the double knockdown of CYP99A2 and CYP99A3 specifically suppresses the elicitor-inducible production of momilactones
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9beta-pimara-7,15-diene + O2 + NADPH + H+
3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide + NADP+ + H2O
show the reaction diagram
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?
syn-pimara-7,15-diene + O2 + NADPH + H+
syn-pimaradien-19-ol + NADP+ + H2O
show the reaction diagram
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?
syn-pimaradien-19-al + O2 + NADPH + H+
syn-pimaradien-19-oate + NADP+ + H2O
show the reaction diagram
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?
syn-pimaradien-19-ol + O2 + NADPH + H+
syn-pimaradien-19-al + NADP+ + H2O
show the reaction diagram
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?
syn-stemod-13(17)-ene + O2 + NADPH + H+
syn-stemoden-19-ol + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
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?
syn-stemoden-19-al + O2 + NADPH + H+
syn-stemoden-19-oate + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
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?
syn-stemoden-19-ol + O2 + NADPH + H+
syn-stemoden-19-al + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
syn-pimara-7,15-diene
pH and temperature not specified in the publication
0.009
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
46
syn-pimara-7,15-diene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
49
syn-stemod-13(17)-ene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23000
syn-pimara-7,15-diene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
41912
5000
syn-stemod-13(17)-ene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
41913
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
expressed in Escherichia coli Rosetta (DE3) cells
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