Information on EC 1.14.13.143 - ent-isokaurene C2-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.13.143
-
RECOMMENDED NAME
GeneOntology No.
ent-isokaurene C2-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-isokaurene + O2 + NADPH + H+ = ent-2alpha-hydroxyisokaurene + H2O + NADP+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Diterpenoid biosynthesis
-
-
oryzalide A biosynthesis
-
-
diterpene phytoalexins precursors biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ent-isokaurene,NADPH:oxygen oxidoreductase (hydroxylating)
This is the initial step in the conversion of ent-isokaurene to the antibacterial oryzalides in rice, Oryza sativa.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
the enzyme acts as a C2-hydroxylase involved in a latter step of phytocassane biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-isokaurene + O2 + NADPH + H+
ent-2alpha-hydroxyisokaurene + H2O + NADP+
show the reaction diagram
-
-
-
-
ir
additional information
?
-
-
the enzyme does not react with ent-cassadiene
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
ent-isokaurene
-
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
ent-isokaurene
Oryza sativa
-
pH and temperature not specified in the publication
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
-