Information on EC 1.14.13.142 - 3-ketosteroid 9alpha-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.142
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RECOMMENDED NAME
GeneOntology No.
3-ketosteroid 9alpha-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
androsta-1,4-diene-3,17-dione + NADH + H+ + O2 = 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
androstenedione degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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Steroid degradation
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androgen and estrogen metabolism
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SYSTEMATIC NAME
IUBMB Comments
androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9alpha-hydroxylating)
The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. It is a two-component system consisting of a terminal oxygenase (KshA) and a ferredoxin reductase (KshB). The oxygenase contains a Rieske-type iron-sulfur center and non-heme iron. The reductase component is a flavoprotein containing an NAD-binding domain and a plant-type iron-sulfur cluster. The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
terminal oxygenase of 3-ketosteroid 9alpha-hydroxylase
UniProt
Manually annotated by BRENDA team
terminal oxygenase of 3-ketosteroid 9alpha-hydroxylase
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-androstadiene-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
1,4-androstadiene-3,17-dione + O2 + NADH + H+
3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione + H2O + NAD+
show the reaction diagram
1-(5alpha)-androstene-3,17-dione + NADH + H+ + O2
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + NAD+ + H2O
show the reaction diagram
1-(5alpha)-androstene-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
1-(5alpha)-androstene-3,17-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
11beta-hydrocortisone + NADH + H+ + O2
9alpha-hydroxy-11beta-hydrocortisone + NAD+ + H2O
show the reaction diagram
11beta-hydrocortisone + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
the wild type homologue KshA5 shows 93% activity compared to 4-androstene-3,17-dione
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?
11beta-hydrocortisone + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
19-nor-4-androstene-3,17-dione + NADH + H+ + O2
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + NAD+ + H2O
show the reaction diagram
19-nor-4-androstene-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
19-nor-4-androstene-3,17-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
23,24-bis-nor cholesta-4-ene-22-oic acid + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
23,24-bis-nor-cholesta-1,4-diene-22-oic acid + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
23,24-bisnorcholesta-4-ene-22-oic acid + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
3-oxo-23,24-bisnorchol-4-en-22-oic acid + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
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?
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA + O2 + NADH + H+
?
show the reaction diagram
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?
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
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?
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA + O2 + NADH + H+
?
show the reaction diagram
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?
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + NADH + H+ + O2
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate + NAD+ + H2O
show the reaction diagram
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA + NADH + H+ + O2
9alpha-hydroxy-3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA + NAD+ + H2O
show the reaction diagram
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?
3-oxo-23,24-bisnorcholesta-4-ene-22-oate + NADH + H+ + O2
3-oxo-9alpha-hydroxy-23,24-bisnorcholesta-4-ene-22-oate + NAD+ + H2O
show the reaction diagram
3-oxo-23,24-bisnorcholesta-4-ene-22-oic acid + NADH + H+ + O2
3-oxo-9alpha-hydroxy-23,24-bisnorcholesta-4-ene-22-oic acid + NAD+ + H2O
show the reaction diagram
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA + NADH + H+ + O2
3-oxo-9alpha-hydroxy-23,24-bisnorcholesta-4-ene-22-oyl-CoA + NAD+ + H2O
show the reaction diagram
3-oxosteroid + NADH + H+ + O2
9alpha-hydroxysteroid + NAD+ + H2O
show the reaction diagram
4-androstene-17beta-ol-3-one + NADH + H+ + O2
9alpha,17beta-dihydroxy-4-androstene-3-one + NAD+ + H2O
show the reaction diagram
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?
4-androstene-17beta-ol-3-one + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
4-androstene-17beta-ol-3-one + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
4-androstene-3,17-dione + NADH + H+ + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
show the reaction diagram
4-androstene-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
4-androstene-3,17-dione + O2 + NADH + H+
9alpha-hydroxy-4-androstene-3,17-dione + H2O + NAD+
show the reaction diagram
4-androstene-3,17-dione + O2 + NADH + H+
9alpha-hydroxyandrosta-4-en-3,17-dione + H2O + NAD+
show the reaction diagram
4-androstene-3,17-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
4-cholestene-3-one + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
4-pregnene-3,20-dione + NADH + H+ + O2
9alpha-hydroxy-4-pregnene-3,20-dione + NAD+ + H2O
show the reaction diagram
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?
4-pregnene-3,20-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
4-pregnene-3,20-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
5alpha-androstane-17beta-ol-3-one + NADH + H+ + O2
9alpha,17beta-dihydroxy-5alpha-androstane-3-one + NAD+ + H2O
show the reaction diagram
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?
5alpha-androstane-17beta-ol-3-one + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
i.e. stanolon, the wild type homologue KshA5 shows 100% activity compared to 4-androstene-3,17-dione
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?
5alpha-androstane-17beta-ol-3-one + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
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?
5alpha-androstane-3,17-dione + NADH + H+ + O2
9alpha-hydroxy-5alpha-androstane-3,17-dione + NAD+ + H2O
show the reaction diagram
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?
5alpha-androstane-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
5alpha-androstane-3,17-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
5beta-androstane-3,17-dione + NADH + H+ + O2
9alpha-hydroxy-5beta-androstane-3,17-dione + NAD+ + H2O
show the reaction diagram
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?
5beta-androstane-3,17-dione + NADH + H+ + O2
? + NAD+ + H2O
show the reaction diagram
5beta-androstane-3,17-dione + O2 + NADH + H+
? + H2O + NAD+
show the reaction diagram
androsta-1,4-diene-3,17-dione + NADH + H+ + O2
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O
show the reaction diagram
androsta-1,4-diene-3,17-dione + O2 + NADH + H+
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + H2O + NAD+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA + O2 + NADH + H+
?
show the reaction diagram
P71875, P9WJ93
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?
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA + O2 + NADH + H+
?
show the reaction diagram
P71875, P9WJ93
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?
androsta-1,4-diene-3,17-dione + NADH + H+ + O2
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe-S center
Rieske-type Fe-S cluster, Rieske monooxygenase
[2Fe-2S]-center
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
1,4-Androstadiene-3,17-dione
in 0.1 M potassium phosphate, pH 7.0, at 25°C
0.01
23,24-bisnorcholesta-4-ene-22-oic acid
Km less than 0.01 mM, isoform KshA1, in 50mMTris-HCl buffer (pH 7.0), temperature not specified in the publication; Km less than 0.01 mM, isoform KshA5, in 50 mM Tris-HCl buffer (pH 7.0), temperature not specified in the publication
0.003
3-oxo-23,24-bisnorchol-4-en-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.0068
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.07
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.017
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.07
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
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0.017
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
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0.003
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
0.0068
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
0.024
4-androstene-3,17-dione
0.1 - 1
androsta-1,4-diene-3,17-dione
0.09 - 1.2
O2
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
1,4-Androstadiene-3,17-dione
Mycobacterium tuberculosis
P71875
in 0.1 M potassium phosphate, pH 7.0, at 25°C
0.08
3-oxo-23,24-bisnorchol-4-en-22-oic acid
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.61
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.25
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
2.7
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
0.25
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
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2.7
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
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0.08
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
0.61
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
0.07
4-androstene-3,17-dione
0.8
androsta-1,4-diene-3,17-dione
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C; pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
2.5 - 7
O2
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.6
1,4-Androstadiene-3,17-dione
Mycobacterium tuberculosis
P71875
in 0.1 M potassium phosphate, pH 7.0, at 25°C
5451
30
3-oxo-23,24-bisnorchol-4-en-22-oic acid
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
41908
90
3-oxo-23,24-bisnorchol-4-en-22-oic acid-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
41910
3.5
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
41909
160
3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
in 0.1 M potassium phosphate, pH 7.0, at 22°C
41911
3.57
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
194803
158.8
3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
194805
26.67
3-oxo-23,24-bisnorcholesta-4-ene-22-oate
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
194802
89.71
3-oxo-23,24-bisnorcholesta-4-ene-22-oyl-CoA
Mycobacterium tuberculosis
P71875, P9WJ93
pH 7.0, 22°C, KshAB; pH 7.0, 22°C, KshAB
194804
2.917 - 3
4-androstene-3,17-dione
1186
7.27 - 7.6
androsta-1,4-diene-3,17-dione
28534
2.45 - 29
O2
9
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
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x * 45000, SDS-PAGE
45370
x * 45370, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
component KshA, sitting drop vapor diffusion method, using 1.1 M sodium malonate, 0.1 M HEPES, pH 7.0, and 0.5% (v/v) Jeffamine M600
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
copurification of recombinant His-tagged KshA mutants with His-tagged KshB; copurification of recombinant His-tagged KshA mutants with His-tagged KshB; copurification of recombinant His-tagged KshA mutants with His-tagged KshB
Ni2+ affinity column chromatography
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Ni2+-NTA agarose column chromatography and Source15Q column chromatography
recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshB from Escherichia coli strains Bl21(DE3) and C41(DE3)
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA1, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshA5, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. kshA5 supports growth on any of the different classes of steroids tested, consistent with its broad expression induction pattern. Recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshB, recombinant expression of kshB in Escherichia coli strains Bl21(DE3) and C41(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells; expressed in Escherichia coli C41(DE3) cells; expression of chimeric kshA mutant genes, KshA1A5beta and KshA5A1beta, in Escherichia coli, coexpression of the His-tagged KshA mutants with His-tagged KshB, molar ratio of KshA5A1/KshB is 1:8, low expression level of soluble KshA5A1alpha enzyme; expression of chimeric kshA mutant genes, KshA1A5beta and KshA5A1beta, in Escherichia coli, coexpression of the His-tagged KshA mutants with His-tagged KshB, molar ratio of KshA5A1/KshB is 1:8, low expression level of soluble KshA5A1alpha enzyme; expression of chimeric kshA mutant genes, KshA1A5beta and KshA5A1beta, in Escherichia coli, coexpression of the His-tagged KshA mutants with His-tagged KshB, molar ratio of KshA5A1/KshB is 1:8, low expression level of soluble KshA5A1alpha enzyme
the terminal oxygenase component of 3-ketosteroid 9alpha-hydroxylase is expressed in Escherichia coli BL21-lambdaDE3 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of kshA2 is induced in parent strain Rhodococcus erythropolis SQ1 in the presence of androsta-1,4-diene-3,17-dione or 9alpha-hydroxy-4-androstene-3,17-dione or a metabolite thereof
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione; in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol; in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D230E/S232T/F238Y
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA1 shows no 3-ketosteroid 9alpha-hydroxylase activity
D242W
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA1 exhibits 3-ketosteroid 9alpha-hydroxylase activity
E236D/T238S/Y244F
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA5 shows no 3-ketosteroid 9alpha-hydroxylase activity
Q209T/A210G
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA1 exhibits 3-ketosteroid 9alpha-hydroxylase activity
T207V/T209S/Y211F/H213R/S214G/T215Q/G216A
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA5 exhibits 3-ketosteroid 9alpha-hydroxylase activity
V201T/S203T/F205Y/R207H/G208S/Q209T/A210G
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA1 exhibits 3-ketosteroid 9alpha-hydroxylase activity
W248D
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA5 exhibits 3-ketosteroid 9alpha-hydroxylase activity
D230E/S232T/F238Y
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site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
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Q209T/A210G
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site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; the mutant of isoform KshA1 exhibits 3-ketosteroid 9alpha-hydroxylase activity
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V201T/S203T/F205Y/R207H/G208S/Q209T/A210G
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site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
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additional information