Information on EC 1.14.13.139 - 3-hydroxyindolin-2-one monooxygenase

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The expected taxonomic range for this enzyme is: Zea mays

EC NUMBER
COMMENTARY hide
1.14.13.139
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RECOMMENDED NAME
GeneOntology No.
3-hydroxyindolin-2-one monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxyindolin-2-one + NAD(P)H + H+ + O2 = 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
DIBOA-glucoside biosynthesis
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Benzoxazinoid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxyindolin-2-one,NAD(P)H:oxygen oxidoreductase (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one-forming)
The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses). It is a member of the cytochrome P-450 dependent monooxygenases.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxyindolin-2-one + NADPH + H+ + O2
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031 - 0.036
3-Hydroxyindolin-2-one
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
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recombinant enzyme, at pH 7.5 and 25C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain WAT11
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expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
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expressed in Saccharomyces cerevisiae WAT11 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CYP71C1 is expressed at a significant and equal level in 2.5 and 6.5 day old seedling shoots, induced 2.1fold in naphthalic anhydride- and naphthalic anhydride/triasulfuron-treated 2.5 day old seedling shoots
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CYP71C1 is repressed in older naphthalic anhydride and naphthalic anhydride/triasulfuron-treated seedling shoots
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CYP71C1 transcript levels in shoots and roots are unaffected by phenobarbital treatment with or without triasulfuron. The pathogens Erwinia stuartii and Acidovorax avenae induce CYP71C3 transcript 1.9 and 2.5fold, respectively
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